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Botany - Plant Tissue Culture Practice Question Paper

12th Standard EM

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Biology

Time : 01:00:00 Hrs
Total Marks : 35
    5 x 1 = 5
  1. Micro propagation involves

    (a)

    vegetative multiplication of plants by using micro-organisms.

    (b)

    vegetative multiplication of plants by using small explants.

    (c)

    vegetative multiplication of plants by using microspores.

    (d)

    Non-vegetative multiplication of plants by using microspores and megaspores.

  2. Solidifying agent used in plant tissue culture is

    (a)

    Nicotinic acid

    (b)

    Cobaltous chloride

    (c)

    EDTA

    (d)

    Agar

  3. Protoplast are the cells devoid of ___________

    (a)

    Cell wall

    (b)

    Cell membrane

    (c)

    Plasma membrane

    (d)

    both A and B

  4. Assertion (A) : Sterilization helps to overcome microbes.
    Reason (R) : Explants are autoclaved.

    (a)

    Both A and R are correct but R is not a correct explanation to A

    (b)

    R explains A

    (c)

    A is correct R is incorrect

    (d)

    Both A and R incorrect

  5. The term used to define the ability of a cell to generate entire individual is

    (a)

    Pleuripotent

    (b)

    Totipotent

    (c)

    Multipotent

    (d)

    Unipotent

  6. 1 x 1 = 1
  7. Source of agar is ___________.

    ()

    Marine algae (Sea weeds)

  8. 5 x 2 = 10
  9. Write the various steps involved in cell suspension culture.

  10. What is the term totipotency refers to?

  11. Define (a) Callus (b) Embryoids

  12. Name any two widely used cryoprotectants.

  13. What does the term 'Bioethics' refers to?

  14. 3 x 3 = 9
  15. Explain the basic concepts involved in plant tissue culture

  16. Name any three secondary metabolites obtained from plants and mention their medicinal aspects.

  17. Geographical Indication refers to the products confined to a specific geographical origin. Name any three Tamil Nadu products of your knowledge that hold GI tag.

  18. 2 x 5 = 10
  19. Write the protocol for artificial seed preparation.

  20. Prepare a protocol for virus free meristem tip culture.

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