Botany - Principles and Processes of Biotechnology Model Question Paper

12th Standard EM

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Biology

Time : 02:00:00 Hrs
Total Marks : 50
    7 x 1 = 7
  1. Restriction enzymes are

    (a)

    Not always required in genetic engineering

    (b)

    Essential tools in genetic engineering

    (c)

    Nucleases that cleave DNA at specific sites

    (d)

    both b and c

  2. Plasmids are

    (a)

    circular protein molecules

    (b)

    required by bacteria

    (c)

    tiny bacteria

    (d)

    confer resistance to antibiotics

  3. Which of the following one is used as a Biosensors?

    (a)

    Electrophoresis

    (b)

    Bioreactors

    (c)

    Vectors

    (d)

    Electroporation

  4. An analysis of chromosomal DNA using the southern hybridisation technique does not use

    (a)

    Electrophoresis

    (b)

    Blotting

    (c)

    Autoradiography

    (d)

    Polymerase Chain Reaction

  5. Some of the characteristics of Bt cotton are

    (a)

    Long fibre and resistant to aphids

    (b)

    Medium yield, long fibre and resistant to beetle pests

    (c)

    high yield and production of toxic protein crystals which kill dipteran pests.

    (d)

    High yield and resistant to ball worms

  6. Zymology deals with __________

    (a)

    Study of yeast fungus and its practical applications.

    (b)

    Study of fermentation and its uses.

    (c)

    Study of Bioreactors and their construction methodology.

    (d)

    Study of zymase producing microbes and its benefits.

  7. Cohesive ends are _____________

    (a)

    Blunt ends

    (b)

    Flush ends

    (c)

    Sticky ends

    (d)

    Symmetric cuts

  8. 4 x 1 = 4
  9. One gene one enzyme hypothesis

  10. (1)

    Beadle and Tatum

  11. Monoclonal antibodies

  12. (2)

    Kohler and Milstein

  13. First transgenic animal

  14. (3)

    Kary Mullis

  15. Development ofPCR technology

  16. (4)

    Ian Wilmet

    6 x 2 = 12
  17. How do you use the biotechnology in modern practice?

  18. What are the materials used to grow microorganism like Spirulina?

  19. What are the enzymes you can used to cut terminal end and internal phospho di ester bond of nucleotide sequence?

  20. Name the chemicals used in gene transfer.

  21. Define fermentation.

  22. What is Restriction Endonuclease?

  23. 4 x 3 = 12
  24. What do you know about the word pBR332?

  25. Mention the application of Biotechnology.

  26. How will you identify a vectors?

  27. What is SCP? Mention its nutritional value.

  28. 3 x 5 = 15
  29. Compare the various types of Blotting techniques

  30. Write the advantages of herbicide tolerant crops

  31. What is bioremediation? give some examples of bioremediation.

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