Botany - Plant Tissue Culture Book Back Questions

12th Standard EM

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Biology

Time : 00:45:00 Hrs
Total Marks : 30
    5 x 1 = 5
  1. The time duration for sterilization process by using autoclave is ______ minutes and the temperature is _______

    (a)

    10 to 30 minutes and 125° C

    (b)

    15 to 30 minutes and 121° C

    (c)

    15 to 20 minutes and 125° C

    (d)

    10 to 20 minutes and 121° C

  2. Which of the following statement is correct

    (a)

    Agar is not extracted from marine algae such as seaweeds

    (b)

    Callus undergoes differentiation and produces somatic embryoids.

    (c)

    Surface sterilization of explants is done by using mercuric bromide

    (d)

    PH of the culture medium is 5.0 to 6.0

  3. The prevention of large scale loss of biological interity

    (a)

    Biopatent

    (b)

    Bioethics

    (c)

    Biosafety

    (d)

    Biofuel

  4. Cryopreservation means it is a process to preserve plant cells, tissues or organs

    (a)

    at very low temperature by using ether.

    (b)

    at very high temperature by using liquid nitrogen

    (c)

    at very low temperature of -196 by using liquid nitrogen

    (d)

    at very low temperature by using liquid nitrogen

  5. Solidifying agent used in plant tissue culture is

    (a)

    Nicotinic acid

    (b)

    Cobaltous chloride

    (c)

    EDTA

    (d)

    Agar

  6. 3 x 2 = 6
  7. How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

  8. Write the various steps involved in cell suspension culture.

  9. What do you mean Embryoids? Write its application.

  10. 3 x 3 = 9
  11. Give the examples for micro propagation performed plants .

  12. Explain the basic concepts involved in plant tissue culture

  13. Based on the material used, how will you classify the culture technology? Explain it.

  14. 2 x 5 = 10
  15. What do you know about Germplasm conservation. Describe it.

  16. Write the protocol for artificial seed preparation.

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