Botany - Principles and Processes of Biotechnology Book Back Questions

12th Standard EM

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Biology

Time : 00:45:00 Hrs
Total Marks : 30
    5 x 1 = 5
  1. Restriction enzymes are

    (a)

    Not always required in genetic engineering

    (b)

    Essential tools in genetic engineering

    (c)

    Nucleases that cleave DNA at specific sites

    (d)

    both b and c

  2. The process of recombinant DNA technology has the following steps
    I. amplication of the gene
    II. Insertion of recombinant DNA into the host cells
    III. Cutting of DNA at specific location using restriction enzyme .
    IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.

    (a)

    ii, iii, iv, and i

    (b)

    iv, ii, iii, and i

    (c)

    i, ii, iii, and iv

    (d)

    iv, iii, i, and ii

  3. pBR 322, BR stands for

    (a)

    Plasmid Bacterial Recombination

    (b)

    Plasmid Bacterial Replication

    (c)

    Plasmid Boliver and Rodriguez

    (d)

    Plasmid Baltimore and Rodriguez

  4. Which of the following one is used as a Biosensors?

    (a)

    Electrophoresis

    (b)

    Bioreactors

    (c)

    Vectors

    (d)

    Electroporation

  5. Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
    Reason: A gene incorporated in the bacterial chromosomal genome gets atomatically transferred to the cross with which bacterium is associated.

    (a)

    Both assertion and reason are true. But reason is correct explanation of assertion.

    (b)

    Assertion is true, but reason is false.

    (c)

    Assertion is false, but reason is true

    (d)

    Both assertion and reason are false.

  6. 3 x 2 = 6
  7. You are working in a biotechnology lab with a becterium namely E.coli. How will you cut the nucleotide sequence? explain it.

  8. What are the enzymes you can used to cut terminal end and internal phospho di ester bond of nucleotide sequence?

  9. Name the chemicals used in gene transfer.

  10. 3 x 3 = 9
  11. What are restriction enzyme. Mention their type with role in Biotechnology.

  12. Is their any possibilities to transfer a suitable desirable gene to host plant without vector? Justify your answer.

  13. How will you identify a vectors?

  14. 2 x 5 = 10
  15. What is bioremediation? give some examples of bioremediation.

  16. Write the benefits and risk of Genetically Modified Foods.

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