Restriction enzymes are _____.

(a)

Not always required in genetic engineering

(b)

Essential tools in genetic engineering

(c)

Nucleases that cleave DNA at specific sites

(d)

both b and c

Plasmids are ______.

(a)

circular protein molecules

(b)

required by bacteria

(c)

tiny bacteria

(d)

confer resistance to antibiotics

pBR 322, BR stands for ______.

(a)

Plasmid Bacterial Recombination

(b)

Plasmid Bacterial Replication

(c)

Plasmid Boliver and Rodriguez

(d)

Plasmid Baltimore and Rodriguez

Which one of the following is not correct statement

(a)

Ti plasmid causes the bunchy top disease

(b)

Multiple cloning site is known as Polylinker

(c)

Non viral method transfection of Nucleic acid in cell

(d)

Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

An analysis of chromosomal DNA using the southern hybridisation technique does not use _____.

(a)

Electrophoresis

(b)

Blotting

(c)

Autoradiography

(d)

Polymerase Chain Reaction

Which of the following person coined the term biotechnology?

(a)

Ernst Hoppe

(b)

Stanley Cohen

(c)

Ian Wilmet

(d)

Karl Ereky

The bacteria responsible for causing Crown Gall:

(a)

Escherichia coli

(b)

Bacillus subtilis

(c)

Bacillus thuringiensis

(d)

Agrobacterium tumefaciens

pUC 19 is an example for_______

(a)

Shuttle vector

(b)

Expression vector

(c)

Cosmid

(d)

Phagemid vector

Assertion (A) : Pseudomonas putida is utilized in the production of Biological hydrogen.
Reason (R): During photosynthesis, it releases oxygen.

(a)

Both A and R are wrong.

(b)

A is right R is wrong.

(c)

R explains A.

(d)

A and R are right, R does not explain A.

Tetracycline is obtained from_________

(a)

S.nodosus

(b)

S.aureofacins

(c)

S.grises

(d)

P. chryosogenum

How do you use the biotechnology in modern practice?

What are the materials used to grow microorganism like Spirulina?

What is a palindrome sequence?

Expand (i) PEG (ii) PHB

Define Biopiracy.

What do you know about the word pBR332?

Mention the application of Biotechnology.

What are restriction enzyme. Mention their type with role in Biotechnology.

What is SCP? Mention its nutritional value.

Mention any three algal species used for SCP production.

Compare the various types of Blotting techniques

Explain the steps involved in recombinant DNA technology

Explain in detail about various types of direct gene transfer method.