Botany - Principles and Processes of Biotechnology Model Questions

12th Standard EM

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Biology

Time : 01:00:00 Hrs
Total Marks : 50
    10 x 1 = 10
  1. Restriction enzymes are

    (a)

    Not always required in genetic engineering

    (b)

    Essential tools in genetic engineering

    (c)

    Nucleases that cleave DNA at specific sites

    (d)

    both b and c

  2. Plasmids are

    (a)

    circular protein molecules

    (b)

    required by bacteria

    (c)

    tiny bacteria

    (d)

    confer resistance to antibiotics

  3. pBR 322, BR stands for

    (a)

    Plasmid Bacterial Recombination

    (b)

    Plasmid Bacterial Replication

    (c)

    Plasmid Boliver and Rodriguez

    (d)

    Plasmid Baltimore and Rodriguez

  4. Which one of the following is not correct statement

    (a)

    Ti plasmid causes the bunchy top disease

    (b)

    Multiple cloning site is known as Polylinker

    (c)

    Non viral method transfection of Nucleic acid in cell

    (d)

    Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

  5. An analysis of chromosomal DNA using the southern hybridisation technique does not use

    (a)

    Electrophoresis

    (b)

    Blotting

    (c)

    Autoradiography

    (d)

    Polymerase Chain Reaction

  6. Which of the following person coined the term biotechnology?

    (a)

    Ernst Hoppe

    (b)

    Stanley Cohen

    (c)

    Ian Wilmet

    (d)

    Karl Ereky

  7. Cohesive ends are _____________

    (a)

    Blunt ends

    (b)

    Flush ends

    (c)

    Sticky ends

    (d)

    Symmetric cuts

  8. pUC 19 is an example for_______

    (a)

    Shuttle vector

    (b)

    Expression vector

    (c)

    Cosmid

    (d)

    Phagemid vector

  9. Assertion (A) : Pseudomonas putida is utilized in the production of Biological hydrogen.
    Reason (R): During photosynthesis, it releases oxygen.

    (a)

    Both A and R are wrong.

    (b)

    A is right R is wrong.

    (c)

    R explains A.

    (d)

    A and R are right, R does not explain A.

  10. Tetracycline is obtained from_________

    (a)

    S.nodosus

    (b)

    S.aureofacins

    (c)

    S.grises

    (d)

    P. chryosogenum

  11. 5 x 2 = 10
  12. How do you use the biotechnology in modern practice?

  13. What are the materials used to grow microorganism like Spirulina?

  14. What is a palindrome sequence?

  15. Expand (i) PEG (ii) PHB

  16. Define Biopiracy.

  17. 5 x 3 = 15
  18. What do you know about the word pBR332?

  19. Mention the application of Biotechnology.

  20. What are restriction enzyme. Mention their type with role in Biotechnology.

  21. What is SCP? Mention its nutritional value.

  22. Mention any three algal species used for SCP production.

  23. 3 x 5 = 15
  24. Compare the various types of Blotting techniques

  25. Describe the steps involved in recombinant DNA technology.

  26. Explain in detail about various types of direct gene transfer method.

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