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12th Standard Bio-Zoology English Medium Applications of Biotechnology Reduced Syllabus Important Questions With Answer Key 2021

12th Standard

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Biology

Time : 01:00:00 Hrs
Total Marks : 100

    Multiple Choice Questions

    15 x 1 = 15
  1. Which one of the following statements is true regarding DNA polymerase used in PCR?

    (a)

    It is used to ligate introduced DNA in recipient cells

    (b)

    It serves as a selectable marker

    (c)

    It is isolated from a Virus

    (d)

    d) It remains active at a high temperature

  2. ELISA is mainly used for

    (a)

    Detection of mutations

    (b)

    Detection of pathogens

    (c)

    Selecting animals having desired traits

    (d)

    Selecting plants having desired traits

  3. Vaccines that use components of a pathogenic organism rather than the whole organism are called

    (a)

    Subunit recombinant vaccines

    (b)

    attenuated recombinant vaccines

    (c)

    DNA vaccines

    (d)

    conventional vaccines

  4. Insulin was first isolated by_______

    (a)

    Banting

    (b)

    Allen

    (c)

    Wilmut

    (d)

    Anderson

  5. Best and Banting isolated insulin from pancreatic islets of___________

    (a)

    E.coli

    (b)

    yeast

    (c)

    dog

    (d)

    pig

  6. Interferons were discovered by________

    (a)

    Banting

    (b)

    Flemming

    (c)

    Engvall

    (d)

    Alick Isaacs and Lindemann

  7. The genes for the formation of the blood dotting factor VIII is located in the_______chromosome.

    (a)

    autosome

    (b)

    X chromosome

    (c)

    allosome

    (d)

    Y chromosome

  8. In the geom line gene therapy, the genes are introduced into the______

    (a)

    bone marrow cells

    (b)

    blood cells

    (c)

    sperms and eggs

    (d)

    skin cells

  9. This type of gene therapy involves the insulin C the anti sense gene which inhibits the expression of the dominant gene

    (a)

    gene inhibition therapy

    (b)

    gene augmentation therapy

    (c)

    germ line gene therapy

    (d)

    somatic cell gene therapy

  10. The purpose of using enzyme in the ELISA test is

    (a)

    to show the coloured product

    (b)

    to show the antigen-antibody reaction

    (c)

    to show the immobilised antigen

    (d)

    to show the immobilized antigen

  11. Throgh PCR technique, amplication of_________ is possible with reverse transcriptase

    (a)

    DNA

    (b)

    rRNA

    (c)

    tRNA

    (d)

    mRNA

  12. ________was the first GI tagged product in India

    (a)

    Kancheepuram silk

    (b)

    Darjeeling tea

    (c)

    Thanjavur paintings

    (d)

    Temple jewellery of Nagarcoil

  13. In genetic engineering, a DNS segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A to D) in this regard and select correct option about which one or more of these can be used as vector/vectors.
    A) A bacterium
    B) Plasmid
    C) Plasmodium
    D) Bacteriophage

    (a)

    (A), (B) and (D) only

    (b)

    (A) only

    (c)

    (A) and (C) only

    (d)

    (B) and (D) only

  14. The first transgenic clone of sheep was called as_________ 

    (a)

    Rosie

    (b)

    Dolly

    (c)

    Sameera

    (d)

    Joel

  15. Name the scientists who discovered Interferons?

    (a)

    Alick Issac and Jean Lindemann

    (b)

    Thalassemia

    (c)

    Haemophilia

    (d)

    Anaemia

  16. 2 Marks

    10 x 2 = 20
  17. Describe the chemical structure of insulin

  18. What is alpha lactalbumin?

  19. Define totipotency.

  20. What is annealing?

  21. Give examples of diseases which can be detected by PCR.

  22. What is BRA!?

  23. Name the two strategies involved in gene therapy

  24. Simply define the PCR technique. Also mention its inventor

  25. What are the Genetically Modified Organisms?

  26. Define Biotechnology.

  27. 3 Marks

    10 x 3 = 30
  28. What are transgenic animals? Give examples

  29. What are DNA vaccines?

  30. Differentiate between Somatic cell gene therapy and germline gene therapy

  31. One of the applications of biotechnology is ‘gene therapy” to treat a person born with a hereditary disease
    i) What does “gene therapy” mean?
    ii) Name the hereditary disease for which the first clinical gene therapy was used.
    iii) Mention the steps involved in gene therapy to treat this disease.

  32. What is gene augmentation therapy?

  33. Briefly explain the structure of insulin

  34. What is ELISA test?

  35. Tbe immune system of a person is suppressed,. In ELISA test, the result is positive
    (I) Name the disease associated with this.
    (ii) Why did he loose his immunity?

  36. Why do children cured by enzyme replacement therapy for ADAdeficiency need periodic treatment? Suggest a permanent solution for this issue.

  37. Whether peR can be done for RNA molecules? Explain.

  38. 5 Marks

    7 x 5 = 35
  39. What are recombinant vaccines?. Explain the types

  40. Mention the advantages and disadvantages of cloning.

  41. Explain the steps involved in the production of recombinant hGH

  42. Explain the creation of Dolly

  43. Explain in detail about stem cell therapy.

  44. List out the uses of Transgenesis

  45. What are the ethical issues about cloning.

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