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12th Standard Biology English Medium Plant Tissue Culture Reduced Syllabus Important Questions With Answer Key 2021

12th Standard

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Biology

Time : 01:00:00 Hrs
Total Marks : 100

    Multiple Choice Questions

    15 x 1 = 15
  1. The time duration for sterilization process by using autoclave is ______ minutes and the temperature is _______

    (a)

    10 to 30 minutes and 125° C

    (b)

    15 to 30 minutes and 121° C

    (c)

    15 to 20 minutes and 125° C

    (d)

    10 to 20 minutes and 121° C

  2. Which of the following statement is correct

    (a)

    Agar is not extracted from marine algae such as seaweeds

    (b)

    Callus undergoes differentiation and produces somatic embryoids.

    (c)

    Surface sterilization of explants is done by using mercuric bromide

    (d)

    PH of the culture medium is 5.0 to 6.0

  3. Select the incorrect statement from given statement

    (a)

    A tonic used for cardiac arrest is obtained from Digitalis purpuria

    (b)

    Medicine used to treat Rheumatic pain is extracted from Capsicum annum

    (c)

    An anti malarial drug is isolated from Cinchona officinalis.

    (d)

    Anti-cancinogenic property is not seen in Catharanthus roseus.

  4. Virus free plants are developed from

    (a)

    Organ culture

    (b)

    Meristem culture

    (c)

    Protoplast culture

    (d)

    Cell suspension culture

  5. The prevention of large scale loss of biological interity

    (a)

    Biopatent

    (b)

    Bioethics

    (c)

    Biosafety

    (d)

    Biofuel

  6. Cryopreservation means it is a process to preserve plant cells, tissues or organs

    (a)

    at very low temperature by using ether.

    (b)

    at very high temperature by using liquid nitrogen

    (c)

    at very low temperature of -196 by using liquid nitrogen

    (d)

    at very low temperature by using liquid nitrogen

  7. The production of secondary metabolites require the use of _________.

    (a)

    Protoplast culture

    (b)

    Organ culture

    (c)

    Cell suspension culture

    (d)

    Virus free germ culture

  8. Protoplast are the cells devoid of ___________

    (a)

    Cell wall

    (b)

    Cell membrane

    (c)

    Plasma membrane

    (d)

    both A and B

  9. Synseeds are developed by encapsulating embryoids with __________.

    (a)

    Sodium chloride

    (b)

    Potassium iodide

    (c)

    Sodium alginate

    (d)

    Potassium dichromate

  10. Identify the correct sequence regarding steps involved in PTC

    (a)

    Sterilization ➝ Incubation ➝ Inoculation  ➝  Embryogenesis ➝ Hardening

    (b)

    Inoculation ➝ Induction ➝ Sterilization ➝ Hardening ➝ Embryogenesis

    (c)

    Induction ➝ Incubation ➝ Inoculation ➝ Hardening ➝ Sterilization

    (d)

    Sterilization ➝ Inoculation ➝ Incubation ➝ Embryogenesis ➝ Hardening

  11. Assertion (A) : Protoplasts are cells devoid of cell wall.
    Reason (R) : Secondary metabolites are synthesized by protoplasmic fusion.

    (a)

    Both A and R are correct but R is not a correct explanation to A

    (b)

    R explains A

    (c)

    A is correct R is incorrect

    (d)

    Both A and R are incorrect

  12. Identify the cryoprotectant

    (a)

    Dimethyl formamide

    (b)

    Fructose

    (c)

    Glycerol

    (d)

    Sodium alginate

  13. Identify the wrong statement:

    (a)

    Artificial seeds are stored for long time under cryopreservation

    (b)

    Somatic embryos are used for artificial seed production

    (c)

    Period of dormancy of artificial seeds is greatly reduced

    (d)

    Encapsulation of embryoids is done using cryoprotectant

  14. The term used to define the ability of a cell to generate entire individual is

    (a)

    Pleuripotent

    (b)

    Totipotent

    (c)

    Multipotent

    (d)

    Unipotent

  15. The phenomenon of reversion of mature cells to meristematic state leading to callus formation is___________

    (a)

    Redifferentiation

    (b)

    Dedifferentiation

    (c)

    either (a) or (b)

    (d)

    none of these

  16. 2 Marks

    10 x 2 = 20
  17. How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

  18. What do you mean Embryoids? Write its application.

  19. Name the four basic concepts of plant tissue culture.

  20. Define sterilization.

  21. Name any four culture media used in plant tissue culture.

  22. Classify plant tissue culture based on types of explants used.

  23. Given below are the secondary metabolites. Mention their plant source.
    (a) Digoxin
    (b) Vincristine.

  24. Define the patent type - Grant.

  25. What does the term 'Bioethics' refers to?

  26. Name the two inherent capacity responsible for cellular totipotency.

  27. 3 Marks

    10 x 3 = 30
  28. Give the examples for micro propagation performed plants .

  29. Based on the material used, how will you classify the culture technology? Explain it.

  30. How the autoclaving is done for culture media?

  31. Point out the factors that determine success rate of tissue culturing.

  32. Mention any three macronutrients and micro nutrients used in MS medium.

  33. How cell suspension is prepared?

  34. What is somatic embryogenesis? Give any two of its applications.

  35. What does the terms specification and claim refers with respect to patents?

  36. Given below are the list of components and accessories used in PTC technique. Sort them out according to their mode of sterilization.
    (a) Glass wares
    (b) Laminar air flow chamber
    (c) Nutrient medium
    (d) Explant

  37. Name the explant through which virus free plantlets can be generated using tissue culturing technique.

  38. 5 Marks

    7 x 5 = 35
  39. Give an account on Cryopreservation.

  40. What do you know about Germplasm conservation. Describe it.

  41. Write the protocol for artificial seed preparation.

  42. Explain the steps involved in protoplast culture.

  43. Point out the applications of plant tissue culture.

  44. Enumerate the advantages of Artificial seeds.

  45. Prepare a protocol for virus free meristem tip culture.

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