Plasmids are ______.

Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases.
Choose the correct option regarding above statements

The process of recombinant DNA technology has the following steps
I. amplication of the gene
II. Insertion of recombinant DNA into the host cells
III. Cutting of DNA at specific location using restriction enzyme .
IV. Isolation of genetic material (DNA) Pick out the correct sequence of step for recombinant DNA technology.

Which one of the following palindromic base sequence in DNA can be easily cut at about the middle by some particular restriction enzymes?

Which of the following one is used as a Biosensors?

Assertion (A): Agrobacterium tumifaciens is popular in genetic engineering because this bacterium is associated with the root nodules of all cereals and pulse crops
Reason(R): A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the cross with which bacterium is associated.

Some of the characteristics of Bt cotton are ______.

Zymology deals with __________

Pick out the mismatched pair(s):
(i) Arnphotericin-B - Streptomyces notatum
(ii) Penicillin - Penicillum nodosus
(iii) Streptomycin - Streptomyces grises
(iv) Tetracycline - Streptomyces aureofacins

Identify the non-fungal species used in SCP production.
(i) Candida
(ii) Chlorella
(iii) Chlamydomonas
(iv) Cellulomonas

Observe the diagram and name A and B.

Statement 1: YAC plasmid behaves like a yeast chromosome.
Statement 2: Circular YAC multiplies in bacteria.

Statement 1: Liposomes are the artificial lipoprotein vesicles.
Statement 2: Liposomes are highly used in gene transfer.

Assertion (A): DMH -11 is a transgenic mustard.
Reason (R): It is developed by using bamase/ barstar technology.

Tetracycline is obtained from_________

How do you use the biotechnology in modern practice?

You are working in a biotechnology lab with a becterium namely E.coli. How will you cut the nucleotide sequence? explain it.

Name the chemicals used in gene transfer.

What are primary metabolites? Give example.

What is Restriction Endonuclease?

What is the role of DNA ligase in genetic engineering?

Point out any two disadvantages of Bt cotton?

What are polylinkers?

Give the tecbnical terminologies for tbe following staements.
(a) Autonomous, self-replicating, circular DNA
(b) Molecular scissors
(c) Symmetrical repreated sequence in DNA strands
(d) Mobile genetic elements

Golden rice is a bio-fortified rice developed by rDNA technology. It differs from its parental strain by possessing 'psy' gene, 'crt-l' gene and 'lyc' gene wbicb are responsible for beta - carotene syntbesis.
(a) Name the sources of the above mentioned genes.
(b) Which disease can be controlled / prevented if a person's diet bas golden rice?

Mention the application of Biotechnology.

What are restriction enzyme. Mention their type with role in Biotechnology.

Mention any three historical events which took place in the 21st century for the development of biotechnology.

Mention any three algal species used for SCP production.

List out the application of single-cell protein.

Give a short note on Alkaline phosphate.

Explain the features of a vector.

Draw and label Ti plasmid.

How does shuttle vectors differ from other types of vectors?

Write a brief note on Biolistics.

Compare the various types of Blotting techniques

What is bioremediation? give some examples of bioremediation.

Write the benefits and risk of Genetically Modified Foods.

Explain in detail about various types of direct gene transfer method.

Write a note on Replica plating technique.

Explain Agarose Gel electrophoresis technique.

Explain the procedure of Southern Blotting Technique. Southern Blotting Techniques - DNA