Zoology - Applications of Biotechnology Model Question Paper

12th Standard EM

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Biology

Time : 01:30:00 Hrs
Total Marks : 50
    7 x 1 = 7
  1. The first clinical gene therapy was done for the treatment of

    (a)

    AIDS

    (b)

    Cancer

    (c)

    Cystic fibrosis

    (d)

    SCID

  2. PCR proceeds in three distinct steps governed by temperature, they are in order of

    (a)

    Denaturation, Annealing, Synthesis

    (b)

    Synthesis, Annealing, Denaturation

    (c)

    Annealing, Synthesis, Denaturation

    (d)

    Denaturation, Synthesis, Annealing

  3. ELISA is mainly used for

    (a)

    Detection of mutations

    (b)

    Detection of pathogens

    (c)

    Selecting animals having desired traits

    (d)

    Selecting plants having desired traits

  4. Recombinant Factor VIII is produced in the ______ cells of the Chinese Hamster

    (a)

    Liver cells

    (b)

    blood cells

    (c)

    ovarian cells

    (d)

    brain cells.

  5. Vaccines that use components of a pathogenic organism rather than the whole organism are called

    (a)

    Subunit recombinant vaccines

    (b)

    attenuated recombinant vaccines

    (c)

    DNA vaccines

    (d)

    conventional vaccines

  6. Alpha lactalbumin is a protein with___________minoacids.

    (a)

    120

    (b)

    133

    (c)

    123

    (d)

    140

  7. Interferons are produced using________

    (a)

    Pigs

    (b)

    yeast

    (c)

    E.coli

    (d)

    viruses

  8. 5 x 1 = 5
  9. PCR

  10. (1)

    Blood cells

  11. GMO

  12. (2)

    RNA amplification

  13. Multipotency

  14. (3)

    Finger printing

  15. PCR

  16. (4)

    Molecular pharming

  17. Edible vaccine

  18. (5)

    Roste

    7 x 2 = 14
  19. Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.

  20. What is genetically engineered Insulin?

  21. Explain how “Rosie” is different from a normal cow

  22. How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?

  23. What is alpha lactalbumin?

  24. What is annealing?

  25. What is GI?

  26. 3 x 3 = 9
  27. What are transgenic animals? Give examples

  28. What are DNA vaccines?

  29. Differentiate between Somatic cell gene therapy and germline gene therapy

  30. 3 x 5 = 15
  31. PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate

  32. Mention the advantages and disadvantages of cloning.

  33. Explain the steps involved in the production of recombinant hGH

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