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Published on: 25/10/2025
Download CBSE Class 12th Standard CBSE Biology question papers, sample papers, important questions, and previous year solved papers in PDF format. Get free study materials, NCERT solutions, and exam preparation resources for Class 12th Standard CBSE Biology
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1.
Why there is a statutory ban on amniocentesis? Why is this technique so named?
2.
How do methanoges help in producing biogas?
3.
Write any four ways used to introduce a desired DNA segment into a bacterial cell i recombinant DNA technology experiments.
4.
Name the plant source of ganja.How does it affect the body of the abuser?
5.
What will be the advantage of making the hybrids into apomicts? Why?
6.
We all must work towards maintaining good health because 'health is wealth'. Enlist any six ways of achieving good health.
7.
(a) Organic farmers prefer biological control of diseases and pests to the use of chemicals for the same purpose. Justify.
(b) Give an example of a bacterium, a fungus and an insect that are used as biocontrol agents.
8.
Why do lepidopterans die when they feed on Bt cotton plant? Explain how does it happen.
9.
The diagram shows the arrangement of blood vessels in the uterus wall and placenta of a pregnant woman.If the women has high blood pressure, would it affect the developing foetus?

10.

(a) Identify the selectable markers in the diagram of E.coli vector shown above.
(b) How is the coding sequence of \(\alpha \)-galactosidase considered a better marker than the ones identified by you in the diagram? Explain.
11.
Mention any six reproduction-related problems/issues.
12.
Write the mode of pollination in Vallisneria and water lily. explain the mechanism of pollination in Vallisneria.
13.
Shilpi heard about clones in cartoon serials on TV and also came to know about "Dolly" as a cloned sheep. She discussed it with her biology teacher who explained the meaning of clone and the method to raise the clones.
Read the above passage and answer the following questions :
(i) What is a clone ?
(ii) How clones are produced in plants ?
(iii) Why did Shilpi discuss the matter with her biology teacher ?
14.
Show with graphic sketch the hormonal control over the menstrual cycle.
15.
Explain the different ways of diagnosing cancer.
16.
17.
18.
19.
20.
21.
22.
To produce insulin via a non-human cell, which of the following would be required to be inserted in the vector is plasmid?
An enzyme
A molecule of insulin
A segment of DNA from another bacterium.
A segment of DNA from a human
23.
The number of meiotic divisions, required to produce 400 seeds in a pea plant is
100
200
400
500
24.
Aedes mosquito is a vector of
Malaria
Filariasis
Cholera
Dengue
25.
Which one of the following helps in the absorption of phosphorus from soil by plants?
Glomus
Rhizobium
Frankia
Anabaena
26.
Which of the following malarial parasites has the longest inculation period?
Plasmodium vivax
Plasmodium falciparum
Plasmodium ovale
Plasmodium malariae
27.
An ovule which becomes curved so that the nucellus and embryo sac lie at right angles to the funicle is
Campylotropous
Anatropous
Orthotropous
Hemitropous
28.
Refer to the diagram given below and answer the following questions.

(i) Identify A.
(ii) State any one property of flowers mentioned in (i).
(iii) How do flowers denoted by A and B differ in carrying out pollination?
(iv) Give one example each of A and B.
(v) In which category (with/without exposed sex organs) will common pansy be placed?
29.
Read the following and answer any four questions from (i) to (v) given below:
Transgenic animals can serve as factories that in some cases, may produce large amount of proteins more efficiently. Transgenic mice have been engineered to express human antibodies by introducing large segment of human DNA encoding human immunoglobulin genes. In transgenic large animals such as cow or sheep proteins of pharmaceutical value can be produced in large quantities in milk which is later purified. Transgenesis can be used to alter many phenotypic properties including growth rate, fat composition, milk production, hair texture, etc.
(i) The production of transgenic animals includes
| (a) identification and separation of desired gene |
| (b) combining the desired gene with appropriate vector |
| (c) introduction of vector in cells, tissues or embryos |
| (d) all of these. |
(ii) In transgenic animals, i.e., cow and sheep proteins of pharmaceutical value are produced in large quantities in the
| (a) blood | (b) accumulated fat | (c) mammary glands | (d) none of these. |
(iii) Mouse is mostly preferred animal for studies on gene transfer because
A. short oestrous cycle
B.long gestation period
C.Short generation time
D.Production of one or two offspring per pregnancy
| (a) both (A) and (C) | (b) both (A) and (B) | (C) Only D | (d) both (C) and (D). |
(iv) Transgenic genes alter many phenotypic properties including
| (a) growth rate | (b) fat composition | (c) milk production | (d) all of these |
(v) Assertion : Transgenic mice have been engineered to express human antibodies.
Reason : Large segment of human DNA encoding human immunoglobulin have been transferred to mice.
| (a) Both assertion and reason are true and reason is the correct explanation of assertion. |
| (b) Both assertion and reason are true but reason is not the correct explanation of assertion. |
| (c) Assertion is true but reason is false. |
| (d) Both assertion and reason are false |
30.
31.
32.
Assertion: A person should be considered reproductively healthy if they have healthy reproductive organs but are emotionally imbalanced.
Reason: This statement about reproductive health was given by WHO.
Codes:
(a) If both Assertion and Reason are true and Reason is the correct explanation of Assertion.
(b) If both Assertion and Reason are true but Reason is not the correct explanation of Assertion.
(c) If Assertion is true but Reason is false.
(d) If both Assertion and Reason are false.
33.
Assertion: Bacteriophage vectors are more advantageous than plasmid vectors.
Reason: Bacteriophage vectors can be easily detected at the time of cloning experiments.
Codes:
(a) Both assertion and reason are true and reason is the correct explanation of assertion.
(b) Both assertion and reason are true but reason is not the correct explanation of assertion.
(c) Assertion is true but reason is false.
(d) Both assertion and reason are false.
1.
Amniocentesis is a technique of foetal sex determination based on the pattern of chromosome, in the amniotic fluid surrounding the developing embryo. A statutory ban has been imposed on this to stop its misuse and therefore to check the increasing cases of female foeticides resulting in the imbalance of sex ratio in human population. It is so called because it is basically a technique of investigating the genetic and metabolic disorders if any of the foetus by taking out a small quantity of amniotic fluid.
2.
1. Methanogens grow anaerobically on celluloric material and produce large amount of methane along with carbon dioxide an hydrogen.
2. Biogas is a mixture of methane and carbon dioxide.
3.
(i) Microinjection
(ii) Biolistics/ gene gun
(iii) Heat-Shock method
(iv) Using disarmed pathogen vectors.
4.
Ganja is obtained from the leaves,flower tops and the resin of the plant Cannabis sativa.
They interact with the cannabinoid receptors present in the brain and affect the cardiovascular system of the body.
5.
In general, the hybrid characters segregate in every generation and are not maintained.
The production of hybrid seed is costly and is too expensive for the farmers.
If the hybrids are made into apomicts, there will be no segregation of characters and seeds can be collected from the plants generation after generation and used for raising the new plants.
6.
(i) Eat healthy food: There are strong links between what we eat and how we feel. A healthy diet is one that has enough essential nutrients, and provides the right amount of calories to maintain a healthy weight.
(ii) Play games and sports: It helps to concentrate, sleep, look, and feel better. Enjoying through games helps in beating stress also.
(iii) Get enough sleep: Every day our body needs time to rest and heal. Sleeping for about eight hours is a must for all, especially for children.
(iv) Do not consume tobacco products or alcohol: Tobacco and alcohol result in illness in proportion to their consumption.
(v) Personal Hygiene
(vi) Proper disposal of waste.
7.
(a) Organic farmers prefer biological control to chemical because:
(i) The chemical methods kill both useful and harmful organism.
(ii) The chemical are extremely harmful to humans and other animals.
(iii) They also pollute the soil and ground water.
(iv) He also believes that eradication of pests is impossible and also it is undesirable because the useful organisms may be dependent on the pests for their food or as host.
(b) Bacterium : Bacillus thuringiensis
Fungus :Trichoderma
Insect : Ladybird beetles feeding on aphids. (or) Dragonflies controlling mosquitoes.
8.
Bt cotton plants are the transgenic plants that express a Bacillus thuringiensis gene called cry gene. This gene, encodes for protein crystals having insecticidal properties against insects of group Lepidoptera, Diptera and Coleoptera. Inside the bacterium, these proteins remain inactive and do not harm the bacteria. However, these inactive crystals can get activated in the alkaline pH of the gut of insects upon ingestion.
After activation, these crystals can bind to the receptors present on the membranes of gut epithelial cells. Due to this binding, the membrane swells and pores are created on them. These pores lead to bursting of cell. Hence, soon the lepidopteran dies due to starvation.
9.
Yes, if a pregnant woman has high blood pressure, the woman develops preeclampsia, which can threaten the lives of both the mother and the foetus.It can cause low birth weight and early delivery of foetus,sometimes the child,born very early also may die.
High blood pressure can harm the mother's kidneys and other organs and also lower the number of platelets.
10.
(a) \({ tet }^{ R },{ amp }^{ R }\)
(b) galactosidase marker differentiates the recombinants from the nonrecombinants based on their ability to produce colour in the presence of a chromogenic substrate.
When a recombinant or an alien DNA is inserted into the coding sequence of the enzyme, there occurs insertional inactivation of the enzyme.
Consequently, a blue colour develops with the chromogenic substrate. when the bacterial colonies do not have an insert (non-recombinants), while no colour develops in the colonies with an insert (recombinants).
Selection of recombinants due to inactivation of antibiotic-resistance is cumbersome because it involves simultaneous pla~ing of two plates having different antibiotics (tetracycline and ampicillin).
11.
(i)Pregnancy
(ii)Delivery
(iii)STDs
(iv)Abortions
(v)Contraception
(vi)Menstrual problems
(vii)Infertility
12.
Vallisneria is pollinated by water.
Water lily is pollinated by insects.
In Vallisneria, the male flowers (later pollen grains) are released on the surface of water and keep floating.
The female flowers reach the surface of water by uncoiling of the long stalk
When the passively carried male flowers/pollen grains meet the female flowers, pollination is effected.
The stalk of the female flower coils and the flower is drawn inside the water column.
13.
(i) Clones are identical copies of an organism produced by biotechnological methods.
(ii) In plants, clones are produced by clonal propagation using tissue culture technique.
(iii) Shilpi was curious to enhance her knowledge about biotechnological techniques.
14.

15.
Cancer-Diagnosis:
Cancer can be diagnosed/detected by:
(i) Biopsy and histopathological studies of the tissue.
(ii) Blood and bone marrow tests for increased cell counts as in leukaemia.
(iii) Use of techniques like radiography,Magnetic Resonance Imagine (MRI) and computed tomography (CT) for cancer of internal organs.
(iv) Use of antibodies against cancer specific antigens.
(v) Applying principles of molecular biology to detect genes in individuals with inherited susceptibility to certain types of cancer.
16.
(c)
17.
(b)
18.
(c)
19.
(c)
20.
(c)
21.
(d)
22.
(d)
A segment of DNA from a human
23.
(d)
500
24.
(d)
Dengue
25.
(a)
Glomus
26.
(d)
Plasmodium malariae
27.
(d)
Hemitropous
28.
(i) 'A' represent chasmogamous flowers.
(ii) These are open flowers with exposed sex organs.
(iii) A (chasmogamous flowers) promote both cross pollination and self-pollination whereas B (cleistogamous flowers) promote self pollination only.
(iv) A-Catharanthus, B-Oxalis
(v) Common pansy, (Viola) is categorised under cleistogamous flowers.
29.
(i) (d)
(ii) (c)
(iii) (a) : Mouse has short gestation period and it produces several number of offspring per pregnancy.
(iv) (d)
(v) (a)
30.
31.
32.
(d) If both Assertion and Reason are false.
Explanation: If both Assertion and Reason are false.
A person is reproductively healthy if he/ she is capable of producing off springs.
33.
(a): Bacteriophage vector have two advantages over plasmid vectors.
(i) They are more efficient than plasmids for the cloning of large DNA fragments. For example, the largest cloned insert in lambda phage is 24kb while for plasmid vector it is less than 15 kb.
(ii) It is easier to screen a large number of phage plaques than bacterial colonies for identification of recombinant vectors.
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