The restriction enzyme EcoRI recognize following palindrome sequence

Transgenic animals are produced for which of the following purposes?
I) To study the normal physiology and development.
II) To study diseases.
III) Toobtain useful biological products.
IV) To test the vaccine safety.
V) To test the chemical safety.
Which of the above statements are correct?

cDNA is synthesized from mRNA by the following enzyme

Which of tho -following genetic disordcr in some children can be cured by bone marrow transplantation?

Mature Insulin consists of

If b represents-> Birth rate
If d represents ->Death rate
If dN represents -> Increase or Decrease in population size
Then, exponential growth is represented by

Identify the DNA segment which is not a palindromic scquence.

Which of the following palindromic base sequences in DNA can be easily cut at abot the middle by some particular restriction enzyme?

The process of recombinant DNA technology has following steps
I. Amplification of gene.
II. Insertion of recombinant DNA into the host cell.
III. Cutting of DNA at specific location using restriction enzyme.
IV. Isolation of genetic material (DNA).
Pick out the option for the correct sequence of step for the process of recombinant DNA technology.

Identify A, B, C and D in the given diagram of E.coli cloning vector pBR322.

Match the terms in Column I with those in Column II.

Column I	Column II
A. Amensalism	1. The interspecific interaction, where both are equally benefitted.
B. Parasitism	2. The interspecific interaction, where one is benefitted and one is neutral
C. Mutualism	3. The interspecific interaction, where one is harmed and the other is neutral.
D. Commensalism	4. The interspecific interaction, where one is benefitted and one is harmed.
E. Competition

The formula of exponential population growth curve, is

The change in population size at a given time interval t, is given by the expression
N_t = N ₀ + B + I - D - E
I, B and D stand respectively for

Assertion : Milk of GM cow(Rosie) was more nutri tionally balanced than natural milk of a cow
Reason : GM cow genome was supplimented by the human gene coding for alpha lact albumin.
Codes:
a) Both (A) and (R)are true and (R) is the correct explanatlon of(A)
b) Both (A) and (R) are true and (R) is not the correct explanation of (A)
c) (A) is true but.(R)is false 
d) Both (A) and(R) are false

Statement I: Predators in nature are 'prudent
Statement II : If a predator is too efficient and overexploits its prey, then the prey might become extinct and following it, the predator will also become extinct for lack of food.
Codes:
a)I is true, but II is false
b)I is false, but II is true
c)Both I and II are true
d)Both I and Il are false

Assertion (A): Lichens represent an intimate mutualistic relationship between a fungus and photosynthesising algae.
Reason (R) : The fungi help the plant in the absorption of essential nutrients from the soil while the plant in turn provides the fungi with energy-yielding carbohydrates.
Codes:
a)If both A and R are true and R is the correct explanation of A
b)If both A and R are true, but R is not the correct explanation of A
c)If A is true, but R is false
d)If A& R are false

Assertion (A) : A population has certain attributes that an individual organism does not.
Reason (R) : An individual may have births and deaths, but a population has birth rates and death rates.
Codes:
a)If both A and R are true and R is the correct explanation of A
b)If both A and R are true, but R is not the correct explanation of A
c)If A is true, but R is false
d)If A& R are false

Statement I: ADA deficiency can be treated by bone marrow transplantation and enzyme replacement therapy
Statement II: The above treatment are completely curative.
Codes:
a) Both statement I& Statement II are correct
b)Both statementI& Statement II are incorrect
c) Statement I is correct & Statement II are incorrect
d) Statement I is incorrect & Statement II are correct

For effective treatment.of a disease, early diagnosis and understanding its pathophysiology is very important. Early diagnosis is not possible with conventional methods such as serum and urine
analysis. Biotechnology has provided us with some techniques such as Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) that serve
the purpose of early diagnosis. Prsence of a pathogen (bacteria, viruses, etc.) is normally suspected only when the pathogen has produced a disease symptom. By this time the concentration of pathogen is already very high in the body. However,m very low concentration of a bacteria or virus (at a time when the symptoms of the disease are not yet visible) can be detected by amplification of their nucleic acid by PCR. PCR is now routinely used to detect HIV in suspected AIDS patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful technique to identify many other genetic disorders. A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridize to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the plhotographic film, bccause thc probe will not have complementarity with the mutated genc. ELISA is based on the principle of antigen-antibody interaction. Infection by pathogen can be detected by the prescnce of antigens (protcins, glycoproteins, ctc.) or by detecting the antibodies synthesized against the pathogen.
On the basis of this information answer the following questions:
1.What is the function of probe?
2.Which technique is routinely used to detect HIV in the AIDS patients?
3.What is the full form of ELISA?
4.When can you suspect presence of pathogen in a person?

Statement I: Exonucleases remove nucleotides from ends of DNA.
Statement Il : Endonucleases make cuts at positions with in the DNA.
Codes
a) Both statement I & Statement II are correct
b) Both statement I& Statement II are incorrect
c) Statement I is correct & Statement II are incorrect
d) Statement I is incorrect & Statement II are Correct

Assertion(A): Anaerobic sludge digester produces "biogas" (inflammable).
Reason(R): In anaerobic sludge digester, bacteria produces a mixture of gases such as methane hydrogen sulphide, and CO, This mixture could be used as a source of energy.
Codes:
1) Both (A) and (R) are true and (R) is the correct explanation of (A)
2) Both (A) and (R) are true and (R) is not the correct explanation of (A)
3) (A) is true but (R) is false
4) Both (A) and (R) are false

Assertion (A): Indirectly, BOD is a measure of organic matter present in the water.

Reason (R): The BOD test measures the rate of uptake of oxygen by microorganisms in a sample of water.

Codes:
(a) Both A and R are true and R is the correct explanation of A.
(b) Both A and R are true and R is not the correct explanation of A.
(c) A is true, but R is false.
(d) A is false, but R is true.

Figure given below representing the insertional inactivation of lac z gene. Study the figure and comment upon the appropriateness of the Assertion and Reason.

Assertion (A) Present of insert in the above figure results in inactivation of enzyme \(\beta\)-galactosidase.
Reason (R) Insertion of recombinant DNA within the coding sequence of \(\beta\)-galactosidase results in colourless colonies.
(a) If both A and R are true and R is the correct explanation of A
(b) If both A and R are true, but R is not the correct explanation of A
(c) If A is true, but R is false
(d) If A is false, but R is true

Assertion : PCR is routinely used for early diagnosis of HIV in suspected AIDS patients.
Reason : PCR can detect low amounts of DNA
Codes :
(a) Both assertion and reason are true and reason is the correct explanation of assertion.
(b) Both assertion and reason are true but reason is not the correct explanation of assertion.
(c) Assertion is true but reason is false.
(d) Both assertion and reason are false