(i) Golden rice is variety of rice in which rice grain has a coating of beta carotene, a precursor of vitamin A.
(ii) It is very useful for poor rice eating human population which generally suffers from vitamin A deficiency.
(iii) Being a farmer, Anil's grandfather was concerned about the latest development in agriculture and wanted to grow such varieties which provide better nutrition contents to human beings.

(i) Restriction Endonucleases.
(ii) Gel Electrophoresis.
(iii) T4 ligase.
(iv) Vector and competant host
(v) DNA delivery system.

Following are the advantages of recombinant Insulin:
(a) Recombinant Insulin can be prepared on a large scale without worrying about the source animal.
(b) Batch to batch consistency can be maintained, and quality can be maintained.
(c) Helps in preventing cruelty against animals.
(d) Chances of allergic reactions have been minimized.
(e) Chances of transmission of infection from source animals have been nullified.
(f) Recombinant Insulin has almost replaced animal-sourced Insulin from around the world.
(g) This paves the way for forms of Insulin, which need not be given through injections.

1. A slaughtered animal produces very little hormone so that the demand was always higher than the supply.
2. It is unethical to slaughter animals for obtaining the drug.
3. Contamination was quite common