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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 1 Marks Questions With Solution 2021

Published on: 29/05/2021

Download CBSE Class 12th Standard CBSE Biology question papers, sample papers, important questions, and previous year solved papers in PDF format. Get free study materials, NCERT solutions, and exam preparation resources for Class 12th Standard CBSE Biology

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Std: 12th Standard CBSE - Biology

chapter: - HOT Questions

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Questions:

1 Marks

9 x 1 = 9

A plasmid without a selectable.marker was chosen as vector for cloning a gene. How does this affect the experiment?

Why are antibiotic-resistance genes used as markers in E.coli?

In making bacteria as a competent host for transformation with r-DNA, it is treated with a specific concentration of divalent cation like calcium. What is the role of calcium?

Any DNA flanked at its two borders by T-DNA can be integrated into the plant genome. Name the vector used for this plant gene transfer?

Retroviruses are disease-causing microorganisms, even then are efficiently used in biotechnology experiments. Explain how is it possible?

Would you choose an exonuclease for producing a recombinant DNA molecule?

Restiction enzymes should not have mora than one site of action in the cloning site of a vector? Comment

Why is it essential to have a 'selectable marker' in a cloning vector?

Why is the enzyme cellulase needed for isolating genetic material from plant cells and not from animal cells?

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Answers Key:

1 Marks

9 x 1 = 9

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In the absence of a selectable marker, it will not be possible to identify the recombinants from the non - recombinants.

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It is because the normal E. coli cells do not have resistance to any such antibiotics.

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Creates pores in its cell wall

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Tumor inducing plasmids (Ti Plasmids)

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Retroviruses in which its disease-causing gene is removed (inactivated) can be used as vectors to deliver the recombinant/alien DNA into animal cells.