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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 1 Marks Questions With Solution 2021

Published on: 25/05/2021

QB365 Provides the updated NCERT Examplar Questions for Class 12 Biology, and also provide the detail solution for each and every ncert examplar questions , QB365 will give all kind of study materials will help to get more marks

Download CBSE Class 12th Standard CBSE Biology question papers, sample papers, important questions, and previous year solved papers in PDF format. Get free study materials, NCERT solutions, and exam preparation resources for Class 12th Standard CBSE Biology

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Std: 12th Standard CBSE - Biology

chapter: - NCERT Exemplers

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Questions:

Generic 2

1 x 2 = 2

How is copy number of the plasmid vector related to yield of recombinant protein?

1 Marks

8 x 1 = 8

Write the significance of adding proteases at the time of isolation of genetic materials.

Name the enzyme responsible for cleavage in the following sequence:
\(\downarrow \)
5' ___ G A A T T C ____3'
3' ___C T T A A G ____5'
\(\uparrow \)

Do molecules (DNA, protein) exhibit biological activity in anhydrous conditions?

Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.

Name a recombinant vaccine that is currently being used in vaccination pro-grammes

What is the significance of adding proteases at the time of isolation of genetic material (DNA)?

What does 'competent' refer to, in competent cells used in transformation experiment?

Would you choose an exonuclease for producing a recombinant DNA molecule?

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Answers Key:

Generic 2

1 x 2 = 2

()
The recombinant DNA can multiply as many times as the copy number of the plasmid vector. thereby determning the yield of recombinant protein So, higher the copy number of the plasmid vector, higher will be the yield of recombinant protein.

1 Marks

8 x 1 = 8

()
Protease is added because it helps in digesting proteins and helps in the breakdown of cell wall by dissolving it.

()
Type II restriction enzyme obtained from Escherichia coli, called EcoRI.

()
DNA and proteins show their activity only in presence of water.

()
If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatement with the restriction enzyme.