The given bioreactor shown in the figure is the simple stirred-tank type bioreactor.
Its purpose is large scale production of recombinant protein or enzymes, using microbial plants, animals and human cells.

The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.

Formation of multiple copies of a particular gene is called gene cloning. A gene is separated and ligated to a vector like plasmid. The recombinant plasmid is introduced into a plasmid free bacterium through transformed bacterium through transformation. The transformed bacterium is made to multiply and form a colony. Each and every bacterium of the colony has a copy of the gene.

1. If denaturation step is missed, the two strands of DNA will not be separated.
2. The primers will not be able to anneal to the template and hence, no extension of the DNA will take place.

This is because plasmid is a circular DNA molecule. When cut with enzyme, it becomes linear, but does not get fragmented.
Whereas, a linear DNA molecule gets cut into two fragments. Hence, a single DNA band is observed for plasmid while, two DNA bands are observed for linear DNA in agarose gel.