Jevan’s friend’s uncle suffers from reduced body weight loss. He suspects HIV. He wants to diagnose immediately. But due to shy he delays to meet doctor. One day Jevan came to know and advices him to go for ELISA or PCR Test. Based on these answer the following questions?
a) What are ELISA and PCR?
b) What makes Jevons uncle feel shy to meet doctor?
c) What are the steps involved in PCR?

Genes encoding resistance to antibiotics like Ampicillin, chloramphenicol, tetracycline are useful selectable markers for E.coli. The normal E.coli cells do not carry resistance against any of these antibiotics. Ankita wants to know more clarity on these scriptures. She asks doubts to her teacher. Based on this please answer the following.
a) Name the best known plasmid vector?
b) What are selectable markers?
c) On what basis Biology teacher advices Ankita.

Sandeep is a Biology Student of class xii. He learnt from his teacher that lack of insulin hormone in our body can be injected through syringes. One day his father shows symptoms of Diabetics and he suggested him to meat doctor for Diagnosing Insulin product. On the basis, Answer the following questions.
a) What did Sandeep understands about insulin?
b) On what basis did Sandeep advice his father?
c) Which American based company produced insulin at first and in which year?

Prabha has seen huge garbage dumps outside your school which are not being regularly disposed of by MCD / Nagar Parishad. Prabha discusses the problems with school mates and decide to organize rally to spread awareness among local people about public hygiene.
A. Prepare two slogans for rally
B. Name any two infectious diseases which may spread due to such unhygienic conditionsat public place

Municipal Corporation has deputed personnel to check for mosquito breeding in your school.
a) Which are the places they should check for mosquitoes and there larvae?
b) Name to diseases which are spread by mosquitoes.
c) Name any two biological agents which can be used to control mosquitoes