Jevan’s friend’s uncle suffers from reduced body weight loss. He suspects HIV. He wants to diagnose immediately. But due to shy he delays to meet doctor. One day Jevan came to know and advices him to go for ELISA or PCR Test. Based on these answer the following questions?
a) What are ELISA and PCR?
b) What makes Jevons uncle feel shy to meet doctor?
c) What are the steps involved in PCR?

In a village Two friend farmers Ramnath and Kishan are cultivating normal cotton saplings. Every year they suffer and get fewer yields and lose money. One day Kishan watches add in television on Bt Cotton and encourages his fellow farmer, they were surprised by seeing high yield. Based on this, answer the following.
a) What is Bt cotton?
b) What way Bt kills lepidopteron, coleopterans and dipterans in the soil?
c) How could the information passed on to the farmers? What is the value that a fellow farmers can learn from this?

Gel Electrophoresis a Technique used to separate DNA fragments after the function of Endonuclease. Sushi couldn’t understand the actual function of Restriction Endonuclease. He takes the help of his friend sham by asking the questions. On the basis answer the following.
a) What is the difference between Exonuclease and Endonuclease?
b) Why sham did help his friend Sushi?
c) How can we see DNA fragments after the separation?

Sandeep is a Biology Student of class xii. He learnt from his teacher that lack of insulin hormone in our body can be injected through syringes. One day his father shows symptoms of Diabetics and he suggested him to meat doctor for Diagnosing Insulin product. On the basis, Answer the following questions.
a) What did Sandeep understands about insulin?
b) On what basis did Sandeep advice his father?
c) Which American based company produced insulin at first and in which year?

Pushpender is a scientist working on GMOs to produce Transgenic Buffaloes which can produce milk of medicinal properties. But he also found adverse effects on which they reduce their life span. Based on this, Answer the following.
a) What values are being neglected by him?
b) Should he continue with transgenic production of Buffaloes?
c) What Reactions and Reflections would he face from various organizations?