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Published on: 28/05/2021
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Questions + Answers key
Take MCQ Biology Test1.
Why is the strand of DNA with 3'-5' polarity transcribed and not the other strand with 5'--> 3' polarity?
2.
Cattle or even human beings, sometimes give to their young ones having extremely different sets of organs like limbs/position of eye(s) etc.Comment with respect of genetics involved in it.
3.
Do you think that the alternate splicing of exons may enable a structural gene to code for several isoproteins from one and the same gene ?If yes.how?If not, why so?
4.
What do you understand by 5'-end and 3'-end?
5.
Here some correct and some wrong statements.Correct only those statements which are wrong:
(a) The genetic code is universal
(b)The genetic code is ambiguous
(c)The genetic code is regenerated.
(d)Transfer RNA(tRNA)carry amino acids to mRNA codons and used again and again in transcription.
(e)UAA, UAG and UGC are terminator codon
(f)Lac operon consists of regulatory genes, operator gene,structural and promoter gene
6.
How many histones make the core part of a nucleosome.What is the basis of binding DNA molecule to the histones?
7.
Calculate the total number of thymine-based present in the double strand DNA if it transcribes a mRNA which reads as follows:
5'-AUGCAUGCAUGCAUGCAGG-3'
8.
A primer comprising of 5 bases is required to allow copying of the following single standard DNA sequence 5'-ATGCCTAGGTC
Name the appropriate primer that should start DNA replication
9.
(a) Name the type of synthesis
(b) Occurrence in the types of synthesis and as shown below:
10.

Why do you see two different types of replicating strands in the given DNA replication fork? Name these strands.
1.
The DNAstrand with 3'-5' polarity only is trancribed and not the other because the RNA-polymerase can polymerize RNA only in 5'-3' direction.
2.
Sometimes cattle or human beings give birth to their young ones that are having different sets of oranges like limbs/position of eye, etc.It happens due to the disturbance in coordinated regulation of expression in sets of genes, which are associated with organ development
3.
1. Functional mRNA of structural genes need not always include all of its exons.This alternate splicing of exons is sex-specific , issue-specific and even development stage-specific.
2. By such alternate splicing of exons, a single gene may encode for several ios proteins and/ or proteins of similar class.In absence of such a kind od splicing, there should have been new genes for every protein/isoprotein.Such an extravagance has been avoided in natural phenomena by alternative splicing.
4.
A polynucleotide chain comprises of a long chain of nucleotides joined by 3'-5' phosphodiester linkages.Such a polymer has at one a free phosphate moiety at 5'-end of ribose sugar.This end of polymer is referred to as 5'-end.Similarly, at the other end of the polymer, the pentose sugar has a free 3'-OH group.This end of polymer is referred to as 3'-end.
5.
(a)Correct
(b)The genetic code is non-ambiguous
(c)The genetic code is degenerate
(d)Transfer RNA(tRNA) carry amino acids to mRNA codons and used again and again in translation
(e)Correct
(f)Correct
6.
The core part of nucleosome comprises of 8 Histone molecules.DNA molecules is negatively charged whereas histone octomer is positively charged.Therefore, negatively charged DNA binds with the positively charged histone octomer
7.
The template strand as 3'-TACGTAGTACGTTAGTCC-5' and the coding strand reads as 5'-ATGCATGCATGCAATCAGG-3' Therefore,the total number of Thymine are=10.
The Carboxyl group(-COOH) of one amino acids reacts with an amino group(-NH2)of other amino acid to form a peptide bond(-CO-NH).Make a peptide bond between the two amino acids by removing water molecule in the given figure


8.
The 3' end of template DNA (parental strand) starts the 5' of the newly synthesised DNA.Therefore, a primer of 5 base required to start DNA replication will be-5'-GACCU
9.
(a) Continuous synthesis of DNA
(b) Discontinuous synthesis of DNA
10.
1.Both the strands of parent DNA function as template strands.
2. On the template strand with \({ 3 }^{ ' }\longrightarrow { 5 }^{ ' }\) polarity, the new strand is synthesised as a continuous stretch as the DNA polymerase can carry out polymerisation of the nucleotides only in \({ 5 }^{ ' }\longrightarrow { 3 }^{ ' }\)direction; this is called continuous synthesis and the strand is called leading strand.
3. On the other template strand with \({ 5 }^{ ' }\longrightarrow { 3 }^{ ' }\) polarity, the new strand is synthesised from the point of replication fork, also in \({ 5 }^{ ' }\longrightarrow { 3 }^{ ' }\) direction, but in short stretches; they are later joined by DNA - ligases to form a strand, called lagging strand.
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