CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 1 Marks Questions 2021
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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 1 Marks Questions 2021
12th Standard CBSE
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Reg.No. :
Biology
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What does 'Hind' and 'III' refer to in the enzyme 'Hind III'?
(a)Hind refers to Haemophilus influenzae strain and III refers to the order of its isolation.
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How is copy number of the plasmid vector related to yield of recombinant protein?
(a)More the copy number o the plasmid/vector, higher will be the number of copies of target DNA.
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Would you choose an exonuclease for producing a recombinant DNA molecule?
(a)No, because exonucleases will remove the nucleotides from the ends o DNA molecule and not at any particular desired region.
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What does 'competent' refer to, in competent cells used in transformation experiment?
(a)Competent refers to the ability of the host cell to take up tha alien or recombinant DNA
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What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
(a)Proteases remove the proteins associated with the DNA.
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Name a recombinant vaccine that is currently being used in vaccination pro-grammes
(a)Hepatitis-B vaccine.
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What modification is done on the Tiplasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
(a)It is modified to be non-pathogenic.
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What is the role of CaCl2 in the preparation of competent cells?
(a)CaCl2 increases the efficiency of the competent cells to pick up DNA through the pores in its cell wall
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Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
(a)If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatement with the restriction enzyme.
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Name the enzyme responsible for cleavage in the following sequence:
\(\downarrow \)
5' ___ G A A T T C ____3'
3' ___C T T A A G ____5'
\(\uparrow \)(a)Type II restriction enzyme obtained from Escherichia coli, called EcoRI.
1 Marks
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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 1 Marks Questions 2021 Answer Keys
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Hind refers to Haemophilus influenzae strain and III refers to the order of its isolation.
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More the copy number o the plasmid/vector, higher will be the number of copies of target DNA.
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No, because exonucleases will remove the nucleotides from the ends o DNA molecule and not at any particular desired region.
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Competent refers to the ability of the host cell to take up tha alien or recombinant DNA
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Proteases remove the proteins associated with the DNA.
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Hepatitis-B vaccine.
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It is modified to be non-pathogenic.
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CaCl2 increases the efficiency of the competent cells to pick up DNA through the pores in its cell wall
-
If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatement with the restriction enzyme.
-
Type II restriction enzyme obtained from Escherichia coli, called EcoRI.
1 Marks