(i) It helps in gender identification.
(ii) It is used to detect the chromosomal aberrations like deletion, duplication, translocation, invasion and nondisjunction of chromosomes.  
(iii) It helps to identify the abnormalities of chromosomes like aneuploidy.
(iv) It is also used in predicting the evolutionary relationships between species.
(v) Genetic diseases in human beings can be detected by this technique.

Sex determination in honeybees
sex determination is as follows
1. Haploid sex determination is seen in honeybees, it is based on the number of sets of chromosome, an individual receives.

2. When the ovum is fertilized by a male gamete the zygote (2n = 32) develops into a female i.e., a queen or worker.
3. When the haploid ovum develops by parthenogenesis. i.e., without fertilization, a male individual, called drone is formed as adult male.
4. The male honeybees (drone) is haploid (with n = 16) and forms sperms by mitosis.

Intersexes: 
1. Intersexes refers to the individuals having the characteristics of both female and male sexes and their sexual anatomy does not seem to fit the typical definition of male or female.
Supersexes: 
1. Supersexes ar formed as a result of abnormal combination of sex chromosomes. 
2. Example : Super males in humans human beings have 44+XYY

1. The X chromosome which is common to male and female carries a number of genes. Such genes are called sex linked genes and the characters controlled by them are termed as sex-linked characters. The inheritance of sex linked genes or traits is known as sex linked inheritance.
2. Sex linked inherited traits are more common in males than females, because males are hemizygous (XY) and therefore express that traits when they inherit one mutant allele.
3. Any male receiving X linked recessive allele from his mother will express the trait as his y  chromosome has no corresponding allele.
4. If a recessive X linked gene causes disorder then more males than females have the disorder because the disorder is caused by a single allele in males. Their y chromosome does not carry any corresponding allele. So that sex linked recessive characters are mole in moles.