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Published on: 13/05/2022
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Questions + Answers key
Take MCQ Biology Test1.
Name the explant through which virus free plantlets can be generated using tissue culturing technique.
2.
Differentiate between Somaclonal and Gametoclonal variations.
3.
How cell suspension is prepared?
4.
Mention any three macronutrients and micro nutrients used in MS medium.
5.
Briefly explain the surface sterilization of explants.
1.
Shoot - tip meristem
2.
Somaclonal variations: Somatic variations found in plants regenerated in vitro (i.e. variations found in leaf, stem, root, tuber or propagule).
Gametoclonal variations: Gametophytic variations found in plants regenerated in vitro gametic origin (i.e. variations found in gametes and gametophytes)
3.
The cell suspension is prepared by transferring a portion of callus to the liquid medium and agitated using rotary shaker instrument. The cells are separated from the callus tissue and used for cell suspension culture.
4.
Macronutrients:
(a) Ammonium nitrate
(b) Potassium nitrate
(c) Calcium chloride
Micronutrients:
(a) Manganese sulphate
(b) Zinc sulphate
(c) Potassium iodide
5.
The plant materials to be used for tissue culture should be surface sterilized by first exposing the material in running tap water and then treating it in surface sterilization agents like 0.1% mercuric chloride, 70% ethanol under aseptic condition inside the Laminar Air Flow Chamber.
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