(i) It helps in gender identification.
(ii) It is used to detect the chromosomal aberrations like deletion, duplication, translocation, invasion and nondisjunction of chromosomes.  
(iii) It helps to identify the abnormalities of chromosomes like aneuploidy.
(iv) It is also used in predicting the evolutionary relationships between species.
(v) Genetic diseases in human beings can be detected by this technique.

Sex determination in honeybees
sex determination is as follows
1. Haploid sex determination is seen in honeybees, it is based on the number of sets of chromosome, an individual receives.

2. When the ovum is fertilized by a male gamete the zygote (2n = 32) develops into a female i.e., a queen or worker.
3. When the haploid ovum develops by parthenogenesis. i.e., without fertilization, a male individual, called drone is formed as adult male.
4. The male honeybees (drone) is haploid (with n = 16) and forms sperms by mitosis.

(i) Certain characters are controlled by nonnuclear genomes found in chloroplast, mitochondria, infective agents and plasmids. These characters do not reveal Mendelian pattern of inheritance.
(ii) The inheritance of the extra chromosomal genes are found to exhibit maternal influence.
(iii) Although both male and female parents contribute equally to the zygote in terms of chromosomal genes, the female parent usually contributes the zygote's initial cytoplasm and organelles, since the sperms contain very little cytoplasm. If there are hereditary units in the cytoplasm, these will be transmitted to the offsprings through the egg, so the offsprings exhibit maternal effect.
(iv) The cytoplasmic extranuclear genes have a characteristic pattern of inheritance which do not resemble the genes of nuclear chromosomes and is known as extra chromosomal or extra nuclear or cytoplasmic inheritance and exhibit maternal influence.
(v) In extra nuclear inheritance, male and female parents contribute equally their nuclear genes to the progeny but do not make equal contribution of' extra chromosomal genes hence, the crosses can yield different (or) non Mendelian results. Extra chromosomal inheritance in Limnaea peregra.
(vi) Limnaea peregra is a freshwater snail. The shell of these animals are spirally coiled. The coiling of the shell is clockwise (dextral) or anticlockwise (sinistral).
(vii) Both type of coilings are produced by two. different types of genetically controlled cleavages namely, dextral cleavage and sinistral cleavage.
(viii) In Limnaea, dextral coiling is normal and Sinistral coiling is a mutant character.
(ix) Direction of coiling is determined by a pair of nuclear genes, D( dextral) and d (sinistral). The gene for dextral (D) being dominant over sinistral coiling (d). In Fig. a dextral snail provides the eggs and a sinistral snail provides the sperm. The offsprings are all dextral (Dd), in the F1 generation.
(x) When the F1 heterozygous dextral individual (Dd) were self crossed the F2 generation showed dextral coiling with genotype of IDD, 2Dd and l dd.
(xi) When a reciprocal cross is made the F1 individuals have Dd genotype but are coiled sinistrally, as in the female parent. In both the crosses the Flare phenotypically similar to the female parent, though the offsprings in both crosses have the same genotype Dd. This is because the genotype of the maternal parent determines the phenotype of the offspring.
(xii) When the F1 sinistral individuals were self crossed, the shell coiling in the F2 generation, were all dextral. This is because the genes do not segregate in the F2 generation. Only in the F3 generation segregation occurs in the ratio of 3 dextral: 1 sinistral.
(xiii) Reasons: The type of cleavage depends on the organization of the egg which is established before the maturation division of the oocyte nucleus and by the influence of the maternal genotype.
(xiv) The direction of coiling of the shell depends upon the orientation of the mitotic spindle during the first cleavage.
(xv) Obviously, maternal control affects only one generation. In each generation the coiling is dependent on the maternal genotype.

(i) The inheritance of a trait that is determined by a gene located on one of the sex chromosomes is called sex linked inheritance.
(ii) Genes present on the differential region of X or Y chromosomes are called sex linked genes. The genes present in the differential region of "X" chromosome are called X linked genes. The X-linked genes have no corresponding alleles in the Y chromosome.
(iii) The genes present in the differential region of Y chromosome are called Y- linked or holandric genes. The Y linked genes have no corresponding allele in X chromosome.
(iv) The Y linked genes inherit along with Y chromosome and they phenotypically express only in the male sex.
(v) Sex linked inherited traits are more common in males than females because, males are hemizygous and therefore express the trait when they inherit one mutant allele.
(vi) The X - linked and Y - linked genes in the differential region (non-homologus region) do not undergo pairing or crossing over during meiosis. The inheritance of X or Y linked genes is called sex-linked inheritance.
X linked Inheritance - Eg. Colour blindness
Y linked Inheritance - Eg. hypertrichosis 

(i) Genic balance mechanisms of sex determination in Drosophila was first studied by C. B. Bridges.
(ii) In Drosophila, the presence of Y chromosome is essential for the fertility of male sex, but does not determine the male sex.
(iii) The gene for femaleness is located on the X chromosome and those for maleness are located on the autosomes.
(iv) When geneticist C. B. Bridges, working with Drosophila, crossed a triploid (3n) female with a normal male, he observed many combinations of autosomes and sex chromosomes in the offspring.
(v) He suggested that sex In Drosophila is determined by the balance between the genes for femaleness located on the 'X' chromosomes and those for maleness located on the 'autosomes". Hence the sex of an individual is determined by the ratio of its X chromosome to that of its autosome sets. This ratio is termed sex index and is expressed as:
\(Sex \ index=\frac { Number \ of \ X \ Chromosomes }{ Number \ of \ Y \ Chromosomes } \left( \cfrac { X }{ A } \right) \)
(vi) Change in this ratio leads to a changed sex phenotype. The results obtained from a cross between triploid female Drosophila (3A:3X) with a diploid male (2A: XY) is shown in below.
(vii) Bridges classical cross of a triploid (3A+XXX) female fly and a diploid (2A+XY) male fly    ♀    ♂
                  Triploid  ♀                  Diploid ♂
Parent            3A + XXX                      2A + XY
Gametes       (2A + XX) (A + X)          (A + X) (A + Y)
                        (2A +X) (A+XX)

Different doses of X chromosomes and autosome sets and their effect in sex determination in Drosphild 

A sex-switch gene in Drosophila directs female development. This gene, Sex-lethal (SxL) located on the X chromosome, has two states of activity. When it is 'on' it directs female development and when it is 'off' maleness ensures. Other genes located on the X chromosome and autosomes regulate this sex-switch gene. However, the Y- chromosome of Drosophila is required for male fertility.