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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes Case Study Questions 2021

Published on: 21/05/2021

QB365 Provides the updated CASE Study Questions for Class 12 Biology, and also provide the detail solution for each and every case study questions . Case study questions are latest updated question pattern from NCERT, QB365 will helps to get more marks in Exams

Download CBSE Class 12th Standard CBSE Biology question papers, sample papers, important questions, and previous year solved papers in PDF format. Get free study materials, NCERT solutions, and exam preparation resources for Class 12th Standard CBSE Biology

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Std: 12th Standard CBSE - Biology

chapter: - Case study Questions

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Questions:

Case Study Questions

5 x 4 = 20

(a) What does the diagram given above, depict?
(b) What is meant by 'largest' and 'smallest' in the diagram'?
(c) Name the compound used to visualise them.
(d) Define elution.

The following diagram illustrates the linking of DNA fragements. Answer the questions that follow the illustration.

(a) Name 'a' and 'b'.
(b) Complete the palindrome, which is recognised by EcoRI.
(c) Name the enzyme that can link the two DNA fragments.

Restriction enzymes typically recognize a symmetrical sequence of DNA.

Notice that the top strand is the same as the bottom strand, but reads backward. When the enzyme cuts the strand between G and A, it leaves overhanging chains:

(a) What is this symmetrical sequence of DNA known as?
(b) What is the significance of these overhanging chains?
(c) Name the restriction enzyme that cuts the strand between G and A.

(a) Name and write the significance of enzyme X.
(b) Name and write the role of enzyme Y.
(c) Name the processes A and B.

(a) Name the particular technique in Biotechnology, whose steps are shown in the figure.
(b) Name the steps 1 to 4 marked in the figure.
(c) Give an example where a human gene product is. obtained from transgenic bacteria.

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Answers Key:

Case Study Questions

5 x 4 = 20

(a) It depicts agarose gel electrophoresis used for separation of DNA fragments.
(b) The 'largest' and 'smallest' mean the DNA fragments oflarge size and small size, respectively.
(c) Ethidium bromide.
(d) Elution is the process in which the separated bands of DNA are cut out from the gel and extracted.

(i) a–Vector DNA
b–Foreign DNA
(ii) 5' — GAATTC — 3'
3' — CTTAAG — 5'
(iii) DNA ligase

(a) It is called a palindromic sequence.
(b) The overhanging chains, called 'sticky ends' facilitate the action of DNA ligase.
(c) EcoRI.

(a) Restriction endonuclease - the same restriction enzyme cuts the foreign DNA and vector/plasmid DNA.
(b) DNA ligase joins the foreign DNA segment to the plasmid.
(c) A is transformation.
B is cell division or clone formation.

(a) Recombinant DN Atechnology/Genetic engineering.
(b) 1. Isolation of the desired segment of DNA.
2. Ligation of the isolated DNA to a plasmid vector.
3. Introduction of rDNA into the bacterial cell.
4. The bacterium produces the gene product.
(c) Human insulin is produced by transgenic Escherichia coli cells.