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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 3 Marks Questions 2021

Published on: 29/05/2021

QB365 Provides the HOT Question Papers for Class 12 Biology, and also provide the detail solution for each and every HOT Questions. HOT Questions will help to get more idea about question pattern in every exams and also will help to get more marks in Exams

Download CBSE Class 12th Standard CBSE Biology question papers, sample papers, important questions, and previous year solved papers in PDF format. Get free study materials, NCERT solutions, and exam preparation resources for Class 12th Standard CBSE Biology

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Std: 12th Standard CBSE - Biology

chapter: - HOT Questions

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Questions:

Generic 3

5 x 3 = 15

Youn have been given a task to break the cells of following organisms and release DNA along with other macromolecules from them. Name the specific enzymes you will select for the task:
(i) Bacterial cell
(ii) Plant cell
(iii) Fungal cell

What will happen if -
(i) A plasmid vector is digested with Eco RI at a single site
(ii) A sample of human DNA is digested with Eco RI.
(iii) The two samples (plasmid and human DNA) are allowed to hybridise in presence of DNA ligase.

Name the method/s by which the transgenic sheeps and goats are made. What are other methods?

Do you see the prospects of viroids being used as plant vectors in near future?

Give an example of a natural form of genetic engineering in which the bacterium inserts gene into plants to cause gall or tumour formation.

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Answers Key:

Generic 3

5 x 3 = 15

(i) Lysozyme for bacterial cell
(ii) Cellulase for plant cell
(iii) Chitinase for fungal cell.

(i) It will produce two sticky ends.
(ii) Human DNA pieces will also form sticky ends.
(iii) Some molecules will form with pieces of human DNA inserted into the plasmid vector at the Eco RI site.

Transgenic sheeps and goats are made by injecting new DNA directly into the nucleus of fertilised eggs. The other methods of delivering new DNA into mammalian cell are ___
(i) by DNA-coated tungsten bullets,
(ii) in a calcium phosphate precipitate and
(iii) by electroporation.

Viroids are smallest and simplest pathogenic agents comprising of 300-4000 bases long, circular, single standed, naked RNA. They are mechanically transferable and infect other parts of the plants. They have capability of being used as plant vectors

The best example of a natural form of genetic engineering is Agrobacterium tumefaciens inserting genes into plants and getting the plants to express those genes in the form of proteins. This infection results in the formation of a gall or tumour in the host.