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Published on: 25/05/2021
QB365 Provides the updated NCERT Examplar Questions for Class 12 Biology, and also provide the detail solution for each and every ncert examplar questions , QB365 will give all kind of study materials will help to get more marks
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Questions + Answers key
Take MCQ Biology Test1.
What does 'Hind' and 'III' refer to in the enzyme 'Hind III'?
2.
What modification is done on the Ti-plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
3.
How is copy number of the plasmid vector related to yield of recombinant protein?
4.
Name the enzyme responsible for cleavage in the following sequence:
\(\downarrow \)
5' ___ G A A T T C ____3'
3' ___C T T A A G ____5'
\(\uparrow \)
5.
Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.
6.
What is the role of CaCl2 in the preparation of competent cells?
7.
Name a recombinant vaccine that is currently being used in vaccination pro-grammes
8.
What is the significance of adding proteases at the time of isolation of genetic material (DNA)?
9.
What does 'competent' refer to, in competent cells used in transformation experiment?
10.
Would you choose an exonuclease for producing a recombinant DNA molecule?
1.
( )
The first letter 'H' indicates the genus of the organism, from which the enzyme was isolaled, i.e. H-genus, Haemophilus.
The roman number (IIl) denotes the sequence in which the restriction endonuclease from that particular genus, species and strain of bacteria have been isolated, i.e. third restriction endonuclease to be isolated from this species.
2.
( )
Tumour inducing (Ti) plasmid of Agrobacterium tumefaciens is modified into a cloning vector by removing/ altering the gene responsible for its pathogenic property.
3.
( )
The recombinant DNA can multiply as many times as the copy number of the plasmid vector. thereby determning the yield of recombinant protein So, higher the copy number of the plasmid vector, higher will be the yield of recombinant protein.
4.
( )
Type II restriction enzyme obtained from Escherichia coli, called EcoRI.
5.
( )
If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatement with the restriction enzyme.
6.
( )
CaCl2 increases the efficiency of the competent cells to pick up DNA through the pores in its cell wall
7.
( )
Hepatitis-B vaccine.
8.
( )
Proteases remove the proteins associated with the DNA.
9.
( )
Competent refers to the ability of the host cell to take up tha alien or recombinant DNA
10.
( )
No, because exonucleases will remove the nucleotides from the ends DNA molecule and not at any particular desired region.
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