Give an example of a natural form of genetic engineering in which the bacterium inserts gene into plants to cause gall or tumour formation.

3

(a)

Do you see the prospects of viroids being used as plant vectors in near future?

3

(a)

Name the method/s by which the transgenic sheeps and goats are made. What are other methods?

3

(a)

What will happen if -
(i) A plasmid vector is digested with Eco RI at a single site
(ii) A sample of human DNA is digested with Eco RI.
(iii) The two samples (plasmid and human DNA) are allowed to hybridise in presence of DNA ligase.

3

(a)

Youn have been given a task to break the cells of following organisms and release DNA along with other macromolecules from them. Name the specific enzymes you will select for the task:
(i) Bacterial cell
(ii) Plant cell
(iii) Fungal cell

3

(a)