Identify the group of scientists who developed the intergenic hybrid - the pomato.

(a)

Yamada et al.

(b)

Horsh et al.

(c)

Takebe et al.

(d)

Melchers et al.

The production of secondary metabolites require the use of _________.

(a)

Protoplast culture

(b)

Organ culture

(c)

Cell suspension culture

(d)

Virus free germ culture

Which of the following condition favours callus induction?

(a)

Temperature of 25°C ± 5°C with 12 hours of photoperiod

(b)

Temperature of 25°C ± 2°C with 18 hours of photoperiod

(c)

Temperature of 25°C ± 5°C with 14 hours of photoperiod

(d)

Temperature of 25°C ± 2°C with 16 hours of photoperiod

Protoplast are the cells devoid of ___________

(a)

Cell wall

(b)

Cell membrane

(c)

Plasma membrane

(d)

both A and B

A widely used fusogen in protoplast culture is ________

(a)

Polymethyl glycol

(b)

Polyethylene glycol

(c)

Polyethylene chloride

(d)

Polyvinyl chloride

Synseeds are developed by encapsulating embryoids with __________.

(a)

Sodium chloride

(b)

Potassium iodide

(c)

Sodium alginate

(d)

Potassium dichromate

The optimal pH of culture medium is generally ___________.

(a)

Acidic

(b)

Basic

(c)

Neutral

(d)

Slightly basic

Identify the correct sequence regarding steps involved in PTC

(a)

Sterilization ➝ Incubation ➝ Inoculation  ➝  Embryogenesis ➝ Hardening

(b)

Inoculation ➝ Induction ➝ Sterilization ➝ Hardening ➝ Embryogenesis

(c)

Induction ➝ Incubation ➝ Inoculation ➝ Hardening ➝ Sterilization

(d)

Sterilization ➝ Inoculation ➝ Incubation ➝ Embryogenesis ➝ Hardening

Dimethyl sulphoxide is a _________.

(a)

Solidifying agent

(b)

Cryoprotectant

(c)

Fusogenic agent

(d)

Stimulant

Assertion (A) : Incubation is followed by Inoculation.
Reason (R) : Explant is inoculated to media.

(a)

Both A and R are correct but R is not a correct explanation to A

(b)

R explains A

(c)

A is correct R is incorrect

(d)

Both A and R are incorrect

Assertion (A) : Sterilization helps to overcome microbes.
Reason (R) : Explants are autoclaved.

(a)

Both A and R are correct but R is not a correct explanation to A

(b)

R explains A

(c)

A is correct R is incorrect

(d)

Both A and R incorrect

Assertion (A) : Protoplasts are cells devoid of cell wall.
Reason (R) : Secondary metabolites are synthesized by protoplasmic fusion.

(a)

Both A and R are correct but R is not a correct explanation to A

(b)

R explains A

(c)

A is correct R is incorrect

(d)

Both A and R are incorrect

Assertion (A) : Development of root from callus is called caulogenesis.
Reason (R) : Caulogenesis is the final step of protoplasmic fusion.

(a)

Both A and R are correct but R is not a correct explanation to A

(b)

R explains A

(c)

A is correct R is incorrect

(d)

Both A and R are incorrect

Assertion (A) : Liquid nitrogen is used in cryopreservation techniques.
Reason (R) : Gene bank DNA bank are the parts of germplasm conservation.

(a)

Both A and R are correct but R is not a correct explanation to A

(b)

R explains A

(c)

A is correct R is incorrect

(d)

Both A and R are incorrect

Identify the cryoprotectant

(a)

Dimethyl formamide

(b)

Fructose

(c)

Glycerol

(d)

Sodium alginate