Which of the following person coined the term biotechnology?

(a)

Ernst Hoppe

(b)

Stanley Cohen

(c)

Ian Wilmet

(d)

Karl Ereky

Zymology deals with __________

(a)

Study of yeast fungus and its practical applications.

(b)

Study of fermentation and its uses.

(c)

Study of Bioreactors and their construction methodology.

(d)

Study of zymase producing microbes and its benefits.

Identify the incorrect statement:

(a)

French chemist Louis Pasteur demonstrated the fermentation.

(b)

Fermentor is a vessel providing optimal condition for microbial action.

(c)

Solvent extraction is an upstream process of fermentation.

(d)

Distillation and filtration comes under down stream process.

Pick out the mismatched pair(s):
(i) Arnphotericin-B - Streptomyces notatum
(ii) Penicillin - Penicillum nodosus
(iii) Streptomycin - Streptomyces grises
(iv) Tetracycline - Streptomyces aureofacins

(a)

i and ii

(b)

ii and iii

(c)

iii and iv

(d)

i only

Identify the non-fungal species used in SCP production.
(i) Candida
(ii) Chlorella
(iii) Chlamydomonas
(iv) Cellulomonas

(a)

i and ii

(b)

ii and iii

(c)

ii, iii and iv

(d)

All the above

Select the correct restriction enzyme which breaks the phosphodiester bond within a DNA molecule.
(i) Ba131
(ii) Hind II
(iii) BamHI
(iv) Pvul

(a)

i and iii

(b)

i, ii and iii

(c)

ii, iii and iv

(d)

i only

The bacteria responsible for causing Crown Gall:

(a)

Escherichia coli

(b)

Bacillus subtilis

(c)

Bacillus thuringiensis

(d)

Agrobacterium tumefaciens

Self-ligation is prevented by____________

(a)

DNA Polymerase

(b)

Helicase

(c)

Alkaline phosphate

(d)

DNA lipase

Observe the diagram and name A and B.

(a)

A - Plasmid B - Vector

(b)

A - Nucleoid - B - Plasmid

(c)

A - Bacterial chromosome B - Vector

(d)

A - Nucleoid - B - x phage DNA

A vector should________
(i) contain suitable marker
(ii) contain ori site
(iii) have poly linkess
(iv) be small in size

(a)

i, ii and iii

(b)

ii, iii and iv

(c)

i, ii and iv

(d)

all the above

Number of base pairs does pBR 322 plasmid contains ______

(a)

322

(b)

4322

(c)

4361

(d)

3264

pUC 19 is an example for_______

(a)

Shuttle vector

(b)

Expression vector

(c)

Cosmid

(d)

Phagemid vector

Statement 1: YAC plasmid behaves like a yeast chromosome.
Statement 2: Circular YAC multiplies in bacteria.

(a)

Statement 1 is correct and Statement 2 is also correct.

(b)

Statement 1 is correct and Statement 2 is incorrect

(c)

Both the statements are incorrect.

(d)

Statement 1 is incorrect and Statement 2 is correct.

Statement 1: Liposomes are the artificial lipoprotein vesicles.
Statement 2: Liposomes are highly used in gene transfer.

(a)

Statement 1 is correct and Statement 2 is also correct.

(b)

Statement 1 is correct and Statement 2 is incorrect.

(c)

Both the statements are incorrect.

(d)

Statement 1 is incorrect and Statement 2 is correct

Statement 1: DNA is a hydrophobic molecule.
Statement 2: T-DNA is a part ofE-coli plasmid.

(a)

Statement 1 is correct and Statement 2 is also correct.

(b)

Statement 1 is correct and Statement 2 is incorrect.

(c)

Both the statements are incorrect.

(d)

Statement 1 is incorrect and Statement 2 is correct.