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12th Standard Biology English Medium Plant Tissue Culture Reduced Syllabus Important Questions 2021

12th Standard

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Biology

Time : 01:00:00 Hrs
Total Marks : 100

    Multiple Choice Questions

    15 x 1 = 15
  1. Virus free plants are developed from

    (a)

    Organ culture

    (b)

    Meristem culture

    (c)

    Protoplast culture

    (d)

    Cell suspension culture

  2. Cryopreservation means it is a process to preserve plant cells, tissues or organs

    (a)

    at very low temperature by using ether.

    (b)

    at very high temperature by using liquid nitrogen

    (c)

    at very low temperature of -196 by using liquid nitrogen

    (d)

    at very low temperature by using liquid nitrogen

  3. Identify the group of scientists who developed the intergenic hybrid - the pomato.

    (a)

    Yamada et al.

    (b)

    Horsh et al.

    (c)

    Takebe et al.

    (d)

    Melchers et al.

  4. The production of secondary metabolites require the use of _________.

    (a)

    Protoplast culture

    (b)

    Organ culture

    (c)

    Cell suspension culture

    (d)

    Virus free germ culture

  5. Protoplast are the cells devoid of ___________

    (a)

    Cell wall

    (b)

    Cell membrane

    (c)

    Plasma membrane

    (d)

    both A and B

  6. A widely used fusogen in protoplast culture is ________

    (a)

    Polymethyl glycol

    (b)

    Polyethylene glycol

    (c)

    Polyethylene chloride

    (d)

    Polyvinyl chloride

  7. Synseeds are developed by encapsulating embryoids with __________.

    (a)

    Sodium chloride

    (b)

    Potassium iodide

    (c)

    Sodium alginate

    (d)

    Potassium dichromate

  8. Identify the correct sequence regarding steps involved in PTC

    (a)

    Sterilization ➝ Incubation ➝ Inoculation  ➝  Embryogenesis ➝ Hardening

    (b)

    Inoculation ➝ Induction ➝ Sterilization ➝ Hardening ➝ Embryogenesis

    (c)

    Induction ➝ Incubation ➝ Inoculation ➝ Hardening ➝ Sterilization

    (d)

    Sterilization ➝ Inoculation ➝ Incubation ➝ Embryogenesis ➝ Hardening

  9. Dimethyl sulphoxide is a _________.

    (a)

    Solidifying agent

    (b)

    Cryoprotectant

    (c)

    Fusogenic agent

    (d)

    Stimulant

  10. Assertion (A) : Development of root from callus is called caulogenesis.
    Reason (R) : Caulogenesis is the final step of protoplasmic fusion.

    (a)

    Both A and R are correct but R is not a correct explanation to A

    (b)

    R explains A

    (c)

    A is correct R is incorrect

    (d)

    Both A and R are incorrect

  11. Assertion (A) : Liquid nitrogen is used in cryopreservation techniques.
    Reason (R) : Gene bank DNA bank are the parts of germplasm conservation.

    (a)

    Both A and R are correct but R is not a correct explanation to A

    (b)

    R explains A

    (c)

    A is correct R is incorrect

    (d)

    Both A and R are incorrect

  12. Identify the cryoprotectant

    (a)

    Dimethyl formamide

    (b)

    Fructose

    (c)

    Glycerol

    (d)

    Sodium alginate

  13. Identify the incorrect statement:
    (a) Exp1ants are surface sterilized
    (b) Nutrient media are autoclaved
    (c) Culture rooms are UV radiated for 15 minutes
    (d) Glasswares and accessories are autoclaved

    (a)

    a only

    (b)

    b and c

    (c)

    d only

    (d)

    none of the above

  14. The phenomenon of reversion of mature cells to meristematic state leading to callus formation is___________

    (a)

    Redifferentiation

    (b)

    Dedifferentiation

    (c)

    either (a) or (b)

    (d)

    none of these

  15. Somatic hybridization is achieved through _________

    (a)

    Protoplast fusion

    (b)

    r-DNA technology

    (c)

    Transformation

    (d)

    Grafting

  16. 2 Marks

    10 x 2 = 20
  17. What is the name of the process given below? Write its 4 types.

  18. What do you mean Embryoids? Write its application.

  19. Define tissue culture.

  20. Define sterilization.

  21. Name any four culture media used in plant tissue culture.

  22. What do you mean by "Hardening" in plant tissue culture technique?

  23. Given below are the secondary metabolites. Mention their plant source.
    (a) Digoxin
    (b) Vincristine.

  24. Define Organogenesis.

  25. Name any two widely used cryoprotectants.

  26. What does ELSI represents to?

  27. 3 Marks

    10 x 3 = 30
  28. Explain the basic concepts involved in plant tissue culture

  29. Compare Redifferentiation with Dedifferentiation.

  30. Mention any three macronutrients and micro nutrients used in MS medium.

  31. How cell suspension is prepared?

  32. What is somatic embryogenesis? Give any two of its applications.

  33. How cryopreservation works?

  34. Comment on Biosafety.

  35. Write any three points that you know about Genetic Engineering Appraisal Committee (GEAC).

  36. Study the process given below and mention the phenomena A and B
    (a) Meristematic tissue \(\overset { A }{ \longrightarrow } \) Permanent tissue
    (b) Callus \(\overset { B }{ \longrightarrow } \) Embryoid

  37. Given below are the list of components and accessories used in PTC technique. Sort them out according to their mode of sterilization.
    (a) Glass wares
    (b) Laminar air flow chamber
    (c) Nutrient medium
    (d) Explant

  38. 5 Marks

    7 x 5 = 35
  39. Give an account on Cryopreservation.

  40. What do you know about Germplasm conservation. Describe it.

  41. Write the protocol for artificial seed preparation.

  42. Explain the steps involved in protoplast culture.

  43. Point out the applications of plant tissue culture.

  44. Discuss the protocol for micropropagation of banana.

  45. Prepare a protocol for virus free meristem tip culture.

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