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12th Standard Biology English Medium principles and processes of Bio-technology Reduced Syllabus Important Questions 2021

12th Standard

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Biology

Time : 01:00:00 Hrs
Total Marks : 100

    Multiple Choice Questions

    10 x 1 = 10
  1. Consider the following statements:
    I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
    II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
    III. Restriction enzymes belongs to a classof enzymes called nucleases.
    C hoose the correct option regarding above statements

    (a)

    I & II

    (b)

    I & III

    (c)

    II & III

    (d)

    I,II & III

  2. `In which techniques Ethidium Bromide is used?

    (a)

    Southern Blotting techniques

    (b)

    Western Blotting techniques

    (c)

    Polymerase Chain Reaction

    (d)

    Agrose Gel Electroporosis

  3. Assertion : Agrobacterium tumifaciens is popular in genetic engineering because this bacteriumis associated with the root nodules of all cereals and pulse crops
    Reason: A gene incorporated in the bacterial chromosomal genome gets atomatically transferred to the cross with which bacterium is associated.

    (a)

    Both assertion and reason are true. But reason is correct explanation of assertion.

    (b)

    Assertion is true, but reason is false.

    (c)

    Assertion is false, but reason is true

    (d)

    Both assertion and reason are false.

  4. Which one of the following is not correct statement

    (a)

    Ti plasmid causes the bunchy top disease

    (b)

    Multiple cloning site is known as Polylinker

    (c)

    Non viral method transfection of Nucleic acid in cell

    (d)

    Polylactic acid is a kind of biodegradable and bioactive thermoplastic.

  5. An analysis of chromosomal DNA using the southern hybridisation technique does not use

    (a)

    Electrophoresis

    (b)

    Blotting

    (c)

    Autoradiography

    (d)

    Polymerase Chain Reaction

  6. An antibiotic gene in a vector usually helps in the selection of

    (a)

    Competent cellsc

    (b)

    Transformed cells

    (c)

    Recombinant cells

    (d)

    None of the above

  7. Which of the following person coined the term biotechnology?

    (a)

    Ernst Hoppe

    (b)

    Stanley Cohen

    (c)

    Ian Wilmet

    (d)

    Karl Ereky

  8. Identify the incorrect statement:

    (a)

    French chemist Louis Pasteur demonstrated the fermentation.

    (b)

    Fermentor is a vessel providing optimal condition for microbial action.

    (c)

    Solvent extraction is an upstream process of fermentation.

    (d)

    Distillation and filtration comes under down stream process.

  9. Select the correct restriction enzyme which breaks the phosphodiester bond within a DNA molecule.
    (i) Ba131
    (ii) Hind II
    (iii) BamHI
    (iv) Pvul

    (a)

    i and iii

    (b)

    i, ii and iii

    (c)

    ii, iii and iv

    (d)

    i only

  10. Statement 1: DNA is a hydrophobic molecule.
    Statement 2: T-DNA is a part ofE-coli plasmid.

    (a)

    Statement 1 is correct and Statement 2 is also correct.

    (b)

    Statement 1 is correct and Statement 2 is incorrect.

    (c)

    Both the statements are incorrect.

    (d)

    Statement 1 is incorrect and Statement 2 is correct.

  11. 2 Marks

    10 x 2 = 20
  12. How do you use the biotechnology in modern practice?

  13. You are working in a biotechnology lab with a becterium namely E.coli. How will you cut the nucleotide sequence? explain it.

  14. What are the enzymes you can used to cut terminal end and internal phospho di ester bond of nucleotide sequence?

  15. How modern biotechnology differs from conventional biotechnology?

  16. Define fermentation.

  17. How microbial enzymes are produced? Mention its significance.

  18. Write an palindrome sequence of DNA.

  19. What is Biopharming?

  20. Define the terms (i) Bioventing (ii) Bioaugmentation

  21. Observe tbe given flow cbart and complete it.

  22. 3 Marks

    10 x 3 = 30
  23. Mention the application of Biotechnology.

  24. What are restriction enzyme. Mention their type with role in Biotechnology.

  25. How will you identify a vectors?

  26. Mention any three historical events which took place in the 21st century for the development of biotechnology.

  27. Provide a stepwise procedure of fermentation process.

  28. What is SCP? Mention its nutritional value.

  29. Explain the procedure behind the naming of Restriction Enzymes by citing an example.

  30. How does shuttle vectors differ from other types of vectors?

  31. Give a brief account on antibiotic resistant marker.

  32. How turmeric biopiracy is prevented by Indian Government?

  33. 5 Marks

    8 x 5 = 40
  34. Compare the various types of Blotting techniques

  35. Write the advantages of herbicide tolerant crops

  36. What is bioremediation? give some examples of bioremediation.

  37. Write the benefits and risk of Genetically Modified Foods.

  38. Describe the steps involved in recombinant DNA technology.

  39. Describe the procedure of Blue- White colony selection methods.

  40. How Agarose Gel Electrophoresis is performed?

  41. Explain the procedure of Southern Blotting Technique. Southern Blotting Techniques - DNA

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