Totipotency refers to _____.

(a)

capacity to generate genetically identical plants.

(b)

capacity to generate a whole plant from any plant cell / explant.

(c)

capacity to generate hybrid protoplasts.

(d)

recovery of healthy plants from diseased plants.

Which of the following statement is correct

(a)

Agar is not extracted from marine algae such as seaweeds

(b)

Callus undergoes differentiation and produces somatic embryoids.

(c)

Surface sterilization of explants is done by using mercuric bromide

(d)

P^H of the culture medium is 5.0 to 6.0

Cryopreservation means it is a process to preserve plant cells, tissues or organs _____.

(a)

at very low temperature by using ether.

(b)

at very high temperature by using liquid nitrogen

(c)

at very low temperature of -196°C by using liquid nitrogen

(d)

at very low temperature by using liquid nitrogen

Which of the following condition favours callus induction?

(a)

Temperature of 25°C ± 5°C with 12 hours of photoperiod

(b)

Temperature of 25°C ± 2°C with 18 hours of photoperiod

(c)

Temperature of 25°C ± 5°C with 14 hours of photoperiod

(d)

Temperature of 25°C ± 2°C with 16 hours of photoperiod

A widely used fusogen in protoplast culture is ________

(a)

Polymethyl glycol

(b)

Polyethylene glycol

(c)

Polyethylene chloride

(d)

Polyvinyl chloride

The optimal pH of culture medium is generally ___________.

(a)

Acidic

(b)

Basic

(c)

Neutral

(d)

Slightly basic

Assertion (A) : Development of root from callus is called caulogenesis.
Reason (R) : Caulogenesis is the final step of protoplasmic fusion.

(a)

Both A and R are correct but R is not a correct explanation to A

(b)

R explains A

(c)

A is correct R is incorrect

(d)

Both A and R are incorrect

Identify the cryoprotectant

(a)

Dimethyl formamide

(b)

Fructose

(c)

Glycerol

(d)

Sodium alginate

The enzymatic mixture for chemical isolation of protoplast is

(a)

0.5% macrozyme, 2% onozuka cellulase, 13% mannitol

(b)

1.5% macrozyme, 0.5% onozuka cellulase, 12% sorbitol

(c)

2% macrozyme, 0.5% onozuka cellulase, 13% sorbitol

(d)

0.1% macrozyme, 2% onozuka cellulase, 15% mannitol

Identify the mismatched pair:

(a)

Digoxin - Digitalis purpuria

(b)

Codeine - Capsicum annum

(c)

Vincristine - Catharanthus roseus

(d)

Quinine - Cinchona officinalis