Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.

How is the amplification of a gene sample of interest carried out using PCR?

What is genetically engineered Insulin?

Explain how “Rosie” is different from a normal cow

How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?

ELISA is a technique based on the principles of antigen-antibody reactions. Can this technique be used in the molecular diagnosis of a genetic disorder such as Phenylketonuria?

Gene therapy is an attempt to correct a Genetic defect by providing a normal gene into the individual. By this the function can be restored. An alternate method would be to provide gene product known as enzyme replacement therapy, which would also restore the function. Which in your opinion is a better option? Give reasons for your answer.

Give examples of diseases which can be detected by PCR.

Define molecular cloning.

What is bioethics?

What is a patent?

Define Interferons.

Who discovered Interferons? On which basis it was classified?

List out the benefits of recombinant vaccines.

Name the two strategies involved in gene therapy