Zoology - Applications of Biotechnology Book Back Questions

12th Standard EM

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Biology

Time : 00:45:00 Hrs
Total Marks : 30
    5 x 1 = 5
  1. Dolly, the sheep was obtained by a technique known as

    (a)

    Cloning by gene transfer

    (b)

    Cloning without the help of gametes

    (c)

    Cloning by tissue culture of somatic cells

    (d)

    Cloning by nuclear transfer

  2. How many amino acids are arranged in the two chains of Insulin

    (a)

    Chain A has 12 and Chain B has 13

    (b)

    Chain A has 21 and Chain B has 30 amino acids

    (c)

    Chain A has 21 and Chain B has 30 amino acids

    (d)

    Chain A has 12 and chain B has 20 amino acids.

  3. PCR proceeds in three distinct steps governed by temperature, they are in order of

    (a)

    Denaturation, Annealing, Synthesis

    (b)

    Synthesis, Annealing, Denaturation

    (c)

    Annealing, Synthesis, Denaturation

    (d)

    Denaturation, Synthesis, Annealing

  4. ELISA is mainly used for

    (a)

    Detection of mutations

    (b)

    Detection of pathogens

    (c)

    Selecting animals having desired traits

    (d)

    Selecting plants having desired traits

  5. Recombinant Factor VIII is produced in the ______ cells of the Chinese Hamster

    (a)

    Liver cells

    (b)

    blood cells

    (c)

    ovarian cells

    (d)

    brain cells.

  6. 3 x 2 = 6
  7. Explain how “Rosie” is different from a normal cow

  8. How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?

  9. Gene therapy is an attempt to correct a Genetic defect by providing a normal gene into the individual. By this the function can be restored. An alternate method would be to provide gene product known as enzyme replacement therapy, which would also restore the function. Which in your opinion is a better option? Give reasons for your answer.

  10. 3 x 3 = 9
  11. If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?

  12. What are DNA vaccines?

  13. Differentiate between Somatic cell gene therapy and germline gene therapy

  14. 2 x 5 = 10
  15. PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate

  16. Explain the steps involved in the production of recombinant hGH

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