Which is used in molecular genetic engineering?

1

(a)

Tomato

(b)

Tobacco

(c)

Carrot

(d)

Arabidopsis

Viral genome incorporated into host DNA is called

1

(a)

Prophase

(b)

Prophage

(c)

Bacteriophage

(d)

None of these

Who discovered recombinant DNA (rDNA) technology?

1

(a)

Har Gobind Khorana

(b)

James D. Watson

(c)

Stanley Cohen and Herbert Boyer

(d)

Walter Sutton and Avery

(e)

Williams Bateson and Hugo de Vries

The technique of genetic engineering includes

1

(a)

creation of recombinant DNA

(b)

use of gene cloning

(c)

gene transfer

(d)

All of these.

Cohen and Boyer isolated an antibiotic resistance gene, by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance, in the year

1

(a)

1962

(b)

1965

(c)

1972

(d)

1982

State what happens when an alien gene is ligated at Sal I site of pBR 322 plasmid.

1

(a)

The transformant loses the tetracycline resistance.

Biotechnologists refer to Agrobacterium tumefaciens as a natural genetic engineer of plants. Give reasons to support the statement.

1

(a)

When it infects a plant cell it delivers a part of its DNA called 'T-DNA' (or Tumour-inducing (Ti) plasmid) into the plant cell and transforms it into a tumour cell.

What does 'competent' refer to, in competent cells used in transformation experiment?

1

(a)

Competent refers to the ability of the host cell to take up tha alien or recombinant DNA

Name the first restriction endonuclease discovered.

1

(a)

Hind II

Name two commonly used vectors for genetic engineering.

1

(a)

Plasmids and bacteriophages

Name the source of the DNA polymerase used in PCR technique. Mention why it is used.

2

(a)

Name any four restriction enzymes.

2

(a)

What is genetic engineering?

2

(a)

How are 'Sticky ends' formed on a DNA strand? Why are these so called?

2

(a)

Explain in sequence the process of amplification of a gene of interest using polymerase chain reaction.

2

(a)

Describe the naming of the restriction enzymes with an example.

6

(a)

The fungus Penicillium chrysogenum is grown in fermentors on an industrial scale to produce penicillin, using a batch culture system.
Explain why, batch culture rather than continuous culture is used for production of penicillin. 

6

(a)

Draw a labelled diagram of a simple stirred-tank bioreactor and describe its functionating.

6

(a)

What are bioreactors? Sketch the two types of bioreactors. What is the utility? Which is the common type of bioreactors?

6

(a)

It is possible to produce a human clone. How will the public know that it is a clone?

6

(a)